resumo
Airborne fine particulate matter (PM2.5) has been linked to neurological diseases, but its cellular and metabolic effects remain incompletely understood. This study assessed the cytotoxic and metabolic impact of PM2.5 samples from Sao Paulo, Brazil, on SH-SY5Y neuroblastoma cells. Even at low toxicity levels (IC10-IC30), PM2.5 organic extracts induced apoptosis, increased TNF-alpha secretion, and triggered moderate oxidative responses. Metabolomic analyses revealed a downregulation of energy-producing pathways, including glycolysis and the TCA cycle, along with decreased ATP and phosphocreatine levels. Compensatory adaptations were evident, such as increased proline oxidation, lipid accumulation, and activation of the creatine-phosphocreatine system. One-carbon metabolism was also affected, with changes suggesting suppression of the folate and methionine cycles. Elevated glutathione levels indicated an enhanced antioxidant response. These findings highlight how PM2.5 disrupts neuronal energy homeostasis and redox balance, offering new insights into the cellular mechanisms of air pollution-related neurotoxicity.
palavras-chave
ENERGY-METABOLISM; OXIDATIVE STRESS; NEUROBLASTOMA-CELLS; AIR-POLLUTION; BRAIN; PATHWAYS; EXPOSURE
categoria
Environmental Sciences & Ecology; Pharmacology & Pharmacy; Toxicology
autores
Silva, T; Alves, C; Evtyugina, MG; Dias, AS; Pereira, GM; Vasconcellos, PD; Andrade, MD; Oliveira, H; Duarte, IF
nossos autores
Helena Oliveira
Bolseiro de pós-Doutoramento
Iola Melissa Fernandes Duarte
Investigador PrincipalProjectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This work was developed with support from CICECO-Aveiro Institute of Materials (UIDB/50011/2020, DOI 10.54499/UIDB/50011/2020; UIDP/50011/2020, DOI 10.54499/UIDP/50011/2020; LA/P/0006/2020; DOI 10.54499/LA/P/0006/2020) , UID Centro de Estudos do Ambiente e Mar (CESAM) + LA/P/0094/2020, and UID/50006 Laboratorio Associado para a Quimica Verde-Tecnologias e Processos Limpos, financed by national funds through the FCT/MCTES (PIDDAC) . The NMR spectrometer is part of the National NMR Network (PTNMR) and is partially supported by Infrastructure Project N degrees 022161. P.C. Vasconcellos thanks the National Research Council for the grant CNPq 301503/2018-4. FCT is also acknowledged for the doctoral grant (SFRH/BD/139647/2018) to T.D.S. and the research contracts under Scientific Employment Stimulus to I.F.D. (CEECIND/02387/2018) and H.O. (CEECIND/04050/2017) .