resumo
This study presents the first application of a high-resolution magic angle spinning H-1 NMR approach to elucidate the metabolic effects of a hypercholesterolemic condition and two high-fiber diets based on rye and wheat bread, respectively, in intact pig liver biopsy samples. Standard 1D and spin-echo H-1 spectra were acquired on a total of 20 biopsy samples, and 2D total correlation spectroscopy experiments were carried out on selected samples for assignment of the observed resonances. Principal component analyses and partial least-squares regression discriminant analysis revealed differences in the hepatic lipid content and choline-containing compounds between normal and hypercholesterolemic pigs. In addition, the results demonstrated that the liver metabolite profile of hypercholesterolemic pigs fed a high-fiber rye bread differed from that of pigs fed high-fiber wheat bread with respect to both the lipoprotein fractions and the choline-containing compounds. These findings suggest that earlier reports on high-fiber rye diet-induced effects on plasma HDL/LDL content partially can be ascribed to effects on liver cholesterol metabolism and that the hepatic phospholipase pathways of phosphatidylcholine breakdown are affected by the high-fiber rye diet.
palavras-chave
IOWA WOMENS HEALTH; WHOLE-GRAIN INTAKE; NMR-SPECTROSCOPY; SERUM-CHOLESTEROL; OAT-BRAN; PATTERN-RECOGNITION; LDL CHOLESTEROL; RAT-LIVER; BARLEY; TISSUE
categoria
Chemistry
autores
Bertram, HC; Duarte, IF; Gil, AM; Knudsen, KEB; Laerke, HN
nossos autores
Ana Maria Pissarra Coelho Gil
Professor Associado com Agregação