resumo
The understanding of the molecular-level interactions between biomolecules and ionic liquids (ILs) in aqueous C media is crucial for the optimization of a number of relevant biotechnological processes. In this work, the influence of a series of amino acids oil the liquid-liquid equilibria between 1-butyl-3-methylimidazolium tricyanomethane and water was studied to evaluate the preferential interactions between these three compounds. The solubility effects observed are dependent oil the polarity, size, and charge distribution of the amino acid side chains and are explained ill terms of a refined version of the model proposed earlier (Freire et al. J. Phys. Chem. B 2009, 113, 202; Tome et al. J. Phys. Chem. B 2009, 113, 2815) for ion specific effects oil aqueous solutions of imidazolium-based ILs. Although acting through different mechanisms, salting-in and salting-out phenomena possess a common basis which is the competition between water-amino acid side chain, IL-amino acid side chain, and water-IL interactions. The delicate balance between these interactions is dependent oil the relative affinities of the biomolecules to water molecules or to IL cation and anion and determines the trend and magnitude of the Solubility effect observed.
palavras-chave
MUTUAL SOLUBILITIES; ENZYMATIC RESOLUTION; BIPHASIC SYSTEMS; 298.15 K; WATER; IMIDAZOLIUM; STABILITY; LIPASE; EXTRACTION; TOXICITY
categoria
Chemistry
autores
Tome, LIN; Dominguez-Perez, M; Claudio, AFM; Freire, MG; Marrucho, IM; Cabeza, O; Coutinho, JAP
nossos autores
Ana Filipa M. Cláudio
Bolseiro de pós-Doutoramento
Isabel Maria Delgado Jana Marrucho Ferreira
Professor Catedrático
Luciana Isabel Nabais Tomé
Bolseiro de pós-Doutoramento
Mara Guadalupe Freire Martins
Investigador CoordenadorGrupos
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
agradecimentos
The authors thank financial Support from Fundacao para a Ciencia e a Tecnologia for Project PTDC/EQU-FTT/65252/2006 and for post-doctoral grant SFRH/BPD/44926/2008 of Luciana I. N. Tome.