Extraction of Biomolecules Using Phosphonium-Based Ionic Liquids + K3PO4 Aqueous Biphasic Systems

resumo

Aqueous biphasic systems (ABS) provide an alternative and efficient approach for the extraction, recovery and purification of biomolecules through their partitioning between two liquid aqueous phases. In this work, the ability of hydrophilic phosphonium-based ionic liquids (ILs) to form ABS with aqueous K3PO4 solutions was evaluated for the first time. Ternary phase diagrams, and respective tie-lines and tie-lines length, formed by distinct phosphonium-based ILs, water, and K3PO4 at 298 K, were measured and are reported. The studied phosphonium-based ILs have shown to be more effective in promoting ABS compared to the imidazolium-based counterparts with similar anions. Moreover, the extractive capability of such systems was assessed for distinct biomolecules (including amino acids, food colourants and alkaloids). Densities and viscosities of both aqueous phases, at the mass fraction compositions used for the biomolecules extraction, were also determined. The evaluated IL-based ABS have been shown to be prospective extraction media, particularly for hydrophobic biomolecules, with several advantages over conventional polymer-inorganic salt ABS.

palavras-chave

AQUEOUS BIPHASIC SYSTEMS; MUTUAL SOLUBILITIES; 2-PHASE SYSTEMS; SALTING-OUT; PLUS WATER; PHASE-BEHAVIOR; PROTEINS; EQUILIBRIA; SEPARATION; SOLVENTS

categoria

Biochemistry & Molecular Biology; Chemistry

autores

Louros, CLS; Claudio, AFM; Neves, CMSS; Freire, MG; Marrucho, IM; Pauly, J; Coutinho, JAP

nossos autores

agradecimentos

The authors would like to acknowledge Cytec Industries for providing all of the phosphonium-based ILs used in this work and Fundacao para a Ciencia e a Tecnologia for its financial support through project PTDC/EQU-FTT/65252/2006.

Partilhe este projeto

Publicações similares

Usamos cookies para atividades de marketing e para lhe oferecer uma melhor experiência de navegação. Ao clicar em “Aceitar Cookies” você concorda com nossa política de cookies. Leia sobre como usamos cookies clicando em "Política de Privacidade e Cookies".