Stem Cell Osteogenic Differentiation Probed by Atomic Force Microscopy-Infrared Nanospectroscopy: Sub-cellular Biochemical Characterization

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abstract

Mesenchymal stem cells (MSC) osteodifferentiation is a promising toll in regenerative medicine of bone tissue. However, challenges in bioengineered bone adequacy and biocompatibility are still an issue. Thus, a deeper understanding of MSC osteodifferentiation is crucial. This study aimed to probe sub-cellular events of the differentiation process, using photothermal Atomic Force Microscopy-Infrared (AFM-IR) measurements, comparing both controls and osteogenic differentiation induced human adipose derived MSC (hAMSC), at different time points (0, 6 and 21 days). Spectra were acquired at the new MIRIAM/B22 end station at Diamond Light Source, with a spatial resolution of ca 100 nm and a spectral resolution of 10 cm^(-1). AFM-IR osteodifferentiation monitoring of hAMSC is a pioneering study. It provided both morphological and chemical information, from the AFM topography images and IR nanospectra. The results are expected to contribute to a more effective use of MSC as key players in bone tissue regeneration.

authors

Martins JTS; Nolasco MM; Rodrigues JEA; Martins CB; Graça I; Carvalho ALMB; Bispo DSC; Oliveira MB; Mano JF; Nogueira HIS; Ribeiro-Claro PJA; Marques MPM; Gil AM

our authors

foto Ana Maria Pissarra Coelho Gil

Ana Maria Pissarra Coelho Gil

Associate Professor with Aggregation
foto Daniela Bispo

Daniela Bispo

PhD Student
foto Helena Isabel Seguro Nogueira

Helena Isabel Seguro Nogueira

Assistant Professor with Aggregation
no photo

Inês C. R. Graça

Research Fellowship
foto João A. Rodrigues

João A. Rodrigues

Junior Researcher
foto João Mano

João Mano

Full professor
no photo

João Tomás Silva Martins

MSc Student
foto Mariana Braga de Oliveira

Mariana Braga de Oliveira

Assistant Researcher
foto Mariela Martins Nolasco

Mariela Martins Nolasco

Assistant Researcher
foto Paulo Ribeiro-Claro

Paulo Ribeiro-Claro

Associate Professor with Aggregation

Groups

G1 - Porous Materials and Nanosystems
G5 - Biomimetic, Biological and Living Materials
G6 - Virtual Materials and Artificial Intelligence

Projects

Metabolite-activated 3D stem cell differentiation into bone (BetterBone)

A Metabolomics-guided Bioreactor for Improved Engineered Bone Implants (BioImplant)

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

acknowledgements

BetterBone (2022.04286.PTDC, doi: 10.54499/2022.04286.PTDC) & BIOIMPLANT (PTDC/BTM-ORG/28835/2017), through COMPETE2020/FEDER (POCI-01-0145-FEDER-028835). CICECO-Aveiro Institute of Materials UIDB/50011/2020 (doi: 10.54499/UIDB/50011/2020), UIDP/50011/2020 (doi: 10.54499/UIDP/50011/2020) & LA/P/0006/2020 (doi: 10.54499/LA/P/0006/2020), financed by national funds through the FCT/MCTES (PIDDAC). SFRH/BD/150655/2020 (doi: 10.54499/SFRH/BD/150655/2020, DSB PhD grant). Portuguese National NMR Network (Project Nº022161) through FEDER (COMPETE 2020/POCI/PORL/FCT (PIDDAC). Diamond Light Source is thanked for access

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Publication Details

type
Conference Communication
year
2025

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