|
authors |
Santos, LF; Silva, AS; Mano, JF |
|
nationality |
International |
|
journal |
ACTA BIOMATERIALIA |
|
author keywords |
Complex-shape; magnetic cell sheet; mechanical behavior; tissue engineering; robustness |
|
keywords |
IN-VITRO; STEM-CELLS; SCAFFOLD; DIFFERENTIATION; NANOPARTICLES; BIOMATERIALS; FORCE; CONSTRUCTION; REGENERATION; CARTILAGE |
|
abstract |
The use of cells as building blocks for tissue engineering purposes has been a matter of research in the recent years. Still, the fabrication of complex-shaped 3D-like constructs using living-based materials is hampered through the difficulty in recapitulating the mechanical properties of the native tissues. In an attempt to develop robust tissue-like constructs, it is herein proposed the fabrication of complex-shaped magnetic cell sheets (CSs) with improved mechanical properties for bone TE. Hence, magnetic CSs with versatile shapes and enhanced mechanical performance are fabricated using a pre-osteoblast cell line (MC3T3-E1) through an universal approach that relies on the design of the substrate, cell density and magnetic force. Results show that such magnetic CSs exhibit a Young's modulus similar to those encountered in the soft tissues. The construction of stratified CSs is also explored using MC3T3-E1 and adipose derived stromal cells (ASCs). The role of the pre-osteoblast cell line on ASCs osteogenesis is herein investigated for the first time in layered scaffold-free structures. After 21 days, the level of osteogenic markers in the heterotypic CS (MC3T3-E1:ASCs) is significantly higher than in the homotypic one (ASCs:ASCs), even in the absence of osteogenic differentiation factors. These evidences open new prospects for the creation of mechanically robust, complex, higher-ordered and completely functional 3D cell-based materials that better resemble the native environment of in vivo tissues. (c) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
|
publisher |
ELSEVIER SCI LTD |
|
issn |
1742-7061 |
|
isbn |
1878-7568 |
|
year published |
2020 |
|
volume |
118 |
|
beginning page |
18 |
|
ending page |
31 |
|
digital object identifier (doi) |
10.1016/j.actbio.2020.10.005 |
|
web of science category |
Engineering, Biomedical; Materials Science, Biomaterials |
|
subject category |
Engineering; Materials Science |
|
unique article identifier |
WOS:000593723400002
|
