Augusto Pedro

Junior Researcher

Students supervision

2016 | Joana Antunes | MSc in Environmental Biotechnology | University of Aveiro |
“Production and purification of interferon alpha-2b from Escherichia coli
cultures using alternative platforms” | Cosupervisor; Supervisor: Mara Freire

2015 | Rúben Coval | MSc in Biotechnology | University of Beira Interior |
“Lyophilization and a preliminary thermodynamic characterization of
recombinant SCOMT_His6” | Co-supervisor; Supervisor: Luís Passarinha.

2014 | Diana Oliveira | MSc in Biotechnology | University of Beira Interior |
“Stabilization of COMT by experimental design approaches” | Co-supervisor;
Supervisor: Luís Passarinha.

2015 | Maria João Abrantes | BSc in Bioengineering | University of Beira Interior |
“Reconstituição funcional da isoforma membranar da catecol-O-metiltransferase
humana em sistemas não micelares” | Supervisor.

Lecturing

Sep 2012 – Jan 2016 | Practical Courses “Cellular and Molecular Biology” | BSc,
Department of Chemistry, University of Beira Interior.


Sep 2013 – Jan 2016 | Practical Courses “Biotechnology” | MSc, Department of
Chemistry, University of Beira Interior.

Academic Degrees Jury Participation

2017 “Funcionalização de uma matriz cromatográfica superporosa com um líquido
iónico” | João Martins | MSc in Biotechnology | University of Beira Interior | Main
examiner.


2016 “Biosynthesis optimization of STEAP1 protein in Pichia pastoris X33 MUT+” |
Margarida Santos | MSc in Biochemistry | University of Beira Interior | Main
examiner.


2016 "Isolation and purification of STEAP1 protein fragment in Escherichia Coli cells:
a potential target for prostate cancer" | Jorge Fernandes | MSc in Biomedical
Sciences | University of Beira Interior | Main examiner.


2014 "Biosíntese, purificação e biointeracção da proteína humana recombinante
Tau com inibidores da doença de Alzheimer" | Luís Clara | BSc in
Biotechnology | University of Beira Interior | Main examiner.


2014 "Protótipo de uma mitocôndria artificial para aplicação na doença de
Alzheimer" | Joana Coelho | BSc in Bioengineering | University of Beira Interior
| Main examiner.


2014 "Caraterização biofísica e bioquímica da MBCOMT ligada a um inibidor
farmacológico" | Rita Monteiro | BSc in Bioengineering | University of Beira
Interior | Main examiner.

Ongoing Supervisions

Projects

Publications

Purification of Histidine-Tagged Membrane-Bound Catechol-O-Methyltransferase from Detergent-Solubilized Pichia pastoris Membranes

Pedro AQ, Gonçalves AM, Queiroz JA, Passarinha LA
2018, Chromatographia.

Cholinium-Based Good's Buffers Ionic Liquids as Remarkable Stabilizers and Recyclable Preservation Media for Recombinant Small RNAs

Pedro, AQ; Pereira, P; Quental, MJ; Carvalho, AP; Santos, SM; Queiroz, JA; Sousa, F; Freire, MG
2018, ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 6, 12, 16645-16656.

New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli

Pereira P, Pedro AQ, Queiroz JA, Figueiras AR, Sousa F
2017, Bioengineered.

Biosynthesis and purification of histidine-tagged human soluble catechol- O -methyltransferase

Pedro AQ, Correia FF, Santos FM, Gonçalves AM, Espírito-Santo G, Bonifácio MJ, Queiroz JA, Maia CJ, Passarinha LA
2016, Journal of Chemical Technology & Biotechnology.

Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum

Pereira P, Pedro AQ, Tomás J, Maia CJ, Queiroz JA, Figueiras AR, Sousa F
2016, Applied Microbiology and Biotechnology.

Evaluation of MutS and Mut+ Pichia pastoris Strains for Membrane-Bound Catechol-O-Methyltransferase Biosynthesis

Pedro AQ, Oppolzer D, Bonifácio MJ, Maia CJ, Queiroz JA, Passarinha LA
2015, Applied Biochemistry and Biotechnology.

Enhanced biosynthesis of plasmid DNA from Escherichia coli VH33 using Box–Behnken design associated to aromatic amino acids pathway

Martins LM, Pedro AQ, Oppolzer D, Sousa F, Queiroz JA, Passarinha LA
2015, Biochemical Engineering Journal .

An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures

Pedro AQ, Martins LM, Dias JML, Bonifácio MJ, Queiroz JA, Passarinha LA
2015, Microbial Cell Factories.

Purification of Membrane-Bound Catechol-O-Methyltransferase by Arginine-Affinity Chromatography

Pedro AQ, Pereira P, Bonifácio MJ, Queiroz JA, Passarinha LA
2015, Chromatographia.

Recovery of biological active catechol- O -methyltransferase isoforms from Q-sepharose

Correia FF, Santos FM, Pedro AQ, Bonifácio MJ, Queiroz JA, Passarinha LA
2014, Journal of Separation Science.

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