Extraction and purification of violacein from Yarrowia lipolytica cells using aqueous solutions of surfactants

authors	Kholany, M; Trebulle, P; Martins, M; Ventura, SPM; Nicaud, JM; Coutinho, JAP
nationality	International
journal	JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
author keywords	solid-liquid extraction; aqueous biphasic systems; violacein; surface-active compounds; ionic liquids; Yarrowia lipolytica
keywords	IONIC LIQUIDS; BIPHASIC SYSTEMS; CHROMOBACTERIUM-VIOLACEUM; BIOACTIVE COMPOUNDS; RECOVERY; OPTIMIZATION; SOLVENTS; CAROTENOIDS; DISRUPTION; STEP
abstract	BACKGROUND The demand for colorants from natural bio-based sources is increasing. Violacein is a natural purple-blue hydrophobic pigment with interesting bioactivity whose expression in genetically modified Yarrowia lipolytica production was successfully achieved. RESULTS In this work, several surfactants were tested in the extraction of violacein from Y. lipolytica cells, and the operational conditions were optimized to maximize the extraction yield. After the optimization, the purification of violacein using aqueous biphasic systems (ABS) composed of Tween 20 and cholinium-based ionic liquids (ILs) was pursued. The ABS were characterized and applied in the separation of violacein from contaminant proteins, reaching a maximum selectivity of 155 with violacein being fully concentrated in the Tween 20-rich phase, with 80% of the contaminant proteins present in the extract being removed. CONCLUSION This work led to a conceptual downstream process based on a first step of (solid-liquid) extraction and a second step addressing the separation of violacein from contaminant proteins using ABS. (c) 2019 Society of Chemical Industry
publisher	WILEY
issn	0268-2575
isbn	1097-4660
year published	2020
volume	95
issue	4
beginning page	1126
ending page	1134
digital object identifier (doi)	10.1002/jctb.6297
web of science category	Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical
subject category	Biotechnology & Applied Microbiology; Chemistry; Engineering
unique article identifier	WOS:000504166500001