resumo
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln(3+)-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to similar to 20 degrees and subcellular gradients of similar to 5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
palavras-chave
ION ENERGY-TRANSFER; FLUORESCENT THERMOSENSORS; COORDINATION-COMPOUNDS; GAP ISSUE; THERMOMETRY; SENSORS
categoria
Chemistry; Science & Technology - Other Topics; Materials Science; Physics
autores
Pinol, R; Zeler, J; Brites, CDS; Gu, YY; Tellez, P; Neto, ANC; da Silva, TE; Moreno-Loshuertos, R; Fernandez-Silva, P; Gallego, AI; Martinez-Lostao, L; Martinez, A; Carlos, LD; Millan, A
nossos autores
Albano Neto Carneiro Neto
Investigador Júnior
Carlos Brites
Professor associado
Justyna Anna Zeler
Bolseiro de pós-Doutoramento
Luís Carlos
Professor Catedrático
Thiago Estefane da Silva
Estudante de doutoramentoProjectos
Nanoparticles - based 2D thermal bioimaging technologies - NanoTBTech (NanoTBTech)
SusPhotoSolutions - Soluções Fotovoltaicas Sustentáveis (SUSPHOTOSOLUTIONS)
agradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 and UIDP/50011/2020, financed by Portuguese funds through the FCT/MEC and when appropriate cofinanced by FEDER under the PT2020 Partnership Agreement. Financial support from the project (NanoHeatControl, POCI-01-0145-FEDER-031469), funded by FEDER, through POCI and by Portuguese funds (OE), through FCT/MCTES, by European Union's Horizon 2020 FET Open program under grant agreement nos. 801305 and 829162, Spanish Ministry of Science Innovation and Universities (grant no: PGC2018_095795_B_I00) and Diputacion General de Aragon (E11/17R) are acknowledged. ANCN thanks SusPhotoSolutions project, CENTRO-01-0145FEDER-000005, Portugal, for his grant. The authors acknowledge the use of Servicio General de Apoyo a la InvestigaciOnSAI, Universidad de Zaragoza.