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authors |
Pinol, R; Zeler, J; Brites, CDS; Gu, YY; Tellez, P; Neto, ANC; da Silva, TE; Moreno-Loshuertos, R; Fernandez-Silva, P; Gallego, AI; Martinez-Lostao, L; Martinez, A; Carlos, LD; Millan, A |
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nationality |
International |
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journal |
NANO LETTERS |
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author keywords |
Intracellular temperature mapping; Luminescence thermometry; Thermogenesis; Lanthanide-based polymer nanomicelles |
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keywords |
ION ENERGY-TRANSFER; FLUORESCENT THERMOSENSORS; COORDINATION-COMPOUNDS; GAP ISSUE; THERMOMETRY; SENSORS |
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abstract |
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln(3+)-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to similar to 20 degrees and subcellular gradients of similar to 5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes. |
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publisher |
AMER CHEMICAL SOC |
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issn |
1530-6984 |
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isbn |
1530-6992 |
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year published |
2020 |
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volume |
20 |
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issue |
9 |
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beginning page |
6466 |
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ending page |
6472 |
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digital object identifier (doi) |
10.1021/acs.nanolett.0c02163 |
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web of science category |
Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter |
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subject category |
Chemistry; Science & Technology - Other Topics; Materials Science; Physics |
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unique article identifier |
WOS:000571442000031
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