Selective G-quadruplex binding by oligoarginine-Ru(dppz) metallopeptides

resumo

A set of Ru(II) metallopeptides containing the dppz ligand has been synthesized using SPPS methods. Fluorescence titration studies show that those metallopeptides featuring an octaarginine tail display a large binding preference for DNA G-quadruplex structures over those lacking it, and also that the interplay between the octoarginine functionalization and the ancillary ligand in the complex has an essential role in the recognition process. Furthermore, the oligoarginine metallopeptides are also efficiently internalized, causing cell death with signs of apoptosis.

palavras-chave

METAL-COMPLEXES; DNA-BINDING; RUTHENIUM; INTERCALATORS; SPECTROSCOPY; LIGANDS; CELLS; DPPZ

categoria

Chemistry

autores

Bouzada, D; Salvado, I; Barka, G; Rama, G; Martinez-Costas, J; Lorca, R; Somoza, A; Melle-Franco, M; Vazquez, ME; Lopez, MV

nossos autores

agradecimentos

Financial support from the Spanish grants CTQ2015-70698-R, BFU2013-43513-R and SAF2014-56763-R, the Xunta de Galicia Centro Singular de Investigacion de Galicia accreditation 2016-2019. CICECO - Aveiro Institute of Materials, POCI-01-0145-FEDER-007679 (UID/CTM/50011/2013), the UE ERDF and the Fundacion AECC (IDEAS197VAZQ-Singulares 2014), are acknowledged.

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