Solvatochromic relationship: Prediction of distribution of ionic solutes in aqueous two-phase systems
authors Madeira, PP; Bessa, A; de Banos, DPC; Teixeira, MA; Alvares-Ribeiro, L; Aires-Barros, MR; Rodrigues, AE; Chait, A; Zaslavsky, BY
nationality International
journal JOURNAL OF CHROMATOGRAPHY A
author keywords Aqueous two-phase partitioning; Solvatochromic; Ionic solutes; Descriptors
keywords SOLVATION ENERGY RELATIONSHIP; SOLVENT PROPERTIES; METHYLENE GROUP; PHASES
abstract Partition ratios of several ionic compounds in 20 different polymer/polymer aqueous two-phase systems (ATPS) containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, were determined. The differences between the electrostatic properties of the phases in all the ATPS were estimated from partitioning of the homologous series of dinitrophenylated-amino acids. Also the solvatochromic solvent parameters characterizing the solvent dipolarity/polarizability (pi*), solvent hydrogen-bond donor acidity (a), and solvent hydrogen-bond acceptor basicity (beta) of aqueous media were measured in the coexisting phases of the ATPS. The solute-specific coefficients for the compounds examined were determined by the multiple linear regression analysis using the modified linear solvation energy relationship equation. The minimal number of ATPS necessary for determination of the coefficients was established and 10 ATPS were selected as a reference ATPS set. The solute-specific coefficients values obtained with this reference set of ATPS were used to predict the partition ratios for the compounds in 10 ATPS not included in the reference set. The predicted partition ratios values were compared to those determined experimentally and found to be in good agreement. It is concluded that the presented model of solute-solvent interactions as the driving force for solute partitioning in polymer/polymer ATPS describes experimental observations with 90-95% accuracy. (C) 2012 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE BV
issn 0021-9673
year published 2013
volume 1271
beginning page 10
ending page 16
digital object identifier (doi) 10.1016/j.chroma.2012.11.028
web of science category Biochemical Research Methods; Chemistry, Analytical
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000314016500002
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