Urine Nuclear Magnetic Resonance (NMR) Metabolomics in Age-Related Macular Degeneration


Biofluid biomarkers of age-related macular degeneration (AMD) are still lacking, and their identification is challenging. Metabolomics is well-suited to address this need, and urine is a valuable accessible biofluid. This study aimed to characterize the urinary metabolomic signatures of patients with different stages of AMD and a control group (>50 years). It was a prospective, cross-sectional study, where subjects from two cohorts were included: 305 from Coimbra, Portugal (AMD patients n = 252; controls n = 53) and 194 from Boston, United States (AMD patients n = 147; controls n = 47). For all participants, we obtained color fundus photographs (for AMD staging) and fasting urine samples, which were analyzed using 1H nuclear magnetic resonance (NMR) spectroscopy. Our results revealed that in both cohorts, urinary metabolomic profiles differed mostly between controls and late AMD patients, but important differences were also found between controls and subjects with early AMD. Analysis of the metabolites responsible for these separations revealed that, even though distinct features were observed for each cohort, AMD was in general associated with depletion of excreted citrate and selected amino acids at some stage of the disease, suggesting enhanced energy requirements. In conclusion, NMR metabolomics enabled the identification of urinary signals of AMD and its severity stages, which might represent potential metabolomic biomarkers of the disease.




Biochemistry & Molecular Biology


Lains, I; Duarte, D; Barros, AS; Martins, AS; Carneiro, TJ; Gil, JQ; Miller, JB; Marques, M; Mesquita, TS; Barreto, P; Kim, IK; Cachulo, MD; Vavvas, DG; Carreira, IM; Murta, JN; Silva, R; Miller, JW; Husain, D; Gil, AM

nossos autores


This project was funded by the Fundacao para a Ciencia e a Tecnologia (FCT) (HMSP-ICJ/0006/2013), the Miller Retina Research Fund (MEE) and the Miller Champalimaud Award (MEE). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, FCT ref. UID/CTM/50011/2019 and POCI-01-0145-FEDER-007679, financed by national funds through the FCT/MCTES. AMG and DD acknowledge the Portuguese National NMR Network (RNRMN), supported by FCT funds, and grant SFRH/BD/119509/2016.

Partilhe este projeto

Publicações similares

Usamos cookies para atividades de marketing e para lhe oferecer uma melhor experiência de navegação. Ao clicar em “Aceitar Cookies” você concorda com nossa política de cookies. Leia sobre como usamos cookies clicando em "Política de Privacidade e Cookies".