Designing biopolymer-based artificial peroxidase for oxidative removal of dibenzothiophene from a model diesel fuel
authors Chakraborty, S; Shet, SM; Pereira, MM; Nataraj, SK; Mondal, D
nationality International
journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
author keywords Biopolymer; Polyelectrolyte complex; Artificial enzymes; Peroxidase; Oxidative desulphurization; Fossil fuels
keywords HORSERADISH-PEROXIDASE; DEEP DESULFURIZATION; CO3O4 NANOPARTICLES; COMPLEX-FORMATION; ALGINATE; CHITOSAN; OPTIMIZATION; CATALYSIS; INSIGHTS
abstract Naturally occurring peroxidases are important for living organisms and have manifold utility in industries. However, lack of stability in harsh reaction conditions hinders wide applicability of such enzymes. Thus, suitable alternative is vital which can endure severe reaction conditions. As a substitute of natural peroxidase, herein, biopolymer-based polyelectrolyte complexes (PECs) coordinated with Fen+ is proposed as macromolecular peroxidase mimicking systems. Three PECs were engineered via complexation of protonated chitosan and alginate with Fe2+ (Fe2+-PEC), Fe-3+ (Fe3+-PEC), and Fe3O4 (Fe3O4-PEC), respectively. Computational study showed the Fe3+-PEC was highly stable with abundant electrostatic and intramolecular hydrogen bonding interactions. The versatility of the Fe-PECs as artificial peroxidase biocatalysts was probed by two types of peroxidase assays-ABTS oxidation in buffer systems (pH 4.0 and 7.0) and pyrogallol oxidation in organic solvents (acetonitrile, ethyl acetate and toluene). Overall, Fe3+-PEC showed remarkably high peroxidase activity both in aqueous buffers and in organic solvents, whereas, Fe3O4-PEC showed least catalytic activity. Finally, as a proof of concept, the ability of the biocatalyst to carry out deep oxidative desulphurization was demonstrated envisaging removal of dibenzothiophene from model fossil fuel in a sustainable way. (C) 2021 Published by Elsevier B.V.
publisher ELSEVIER
issn 0141-8130
isbn 1879-0003
year published 2021
volume 183
beginning page 1784
ending page 1793
digital object identifier (doi) 10.1016/j.ijbiomac.2021.05.141
web of science category 10
subject category Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science
unique article identifier WOS:000669499500002
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journal impact factor 5.162
5 year journal impact factor 5.137
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