resumo
Medical adhesives are emerging as an important clinical tool as adjuvants for sutures and staples in wound closure and healing and in the achievement of hemostasis. However, clinical adhesives combining cytocompatibility, as well as strong and stable adhesion in physiological conditions, are still in demand. Herein, a mussel-inspired strategy is explored to produce adhesive coacervates using tannic acid (TA) and methacrylate pullulan (PUL-MA). TA|PUL-MA coacervates mainly comprise van der Waals forces and hydrophobic interactions. The methacrylic groups in the PUL backbone increase the number of interactions in the adhesives matrix, resulting in enhanced cohesion and adhesion strength (72.7 Jm−2), compared to the non-methacrylated coacervate. The adhesive properties are kept in physiologic-mimetic solutions (72.8 Jm−2) for 72 h. The photopolymerization of TA|PUL-MA enables the on-demand detachment of the adhesive. The poor cytocompatibility associated with the use of phenolic groups is here circumvented by mixing reactive oxygen species-degrading enzyme in the adhesive coacervate. This addition does not hamper the adhesive character of the materials, nor their anti-microbial or hemostatic properties. This affordable and straightforward methodology, together with the tailorable adhesivity even in wet environments, high cytocompatibility, and anti-bacterial activity, enables foresee TA|PUL-MA as a promising ready-to-use bioadhesive for biomedical applications.
autores
Margarida M. A. Sacramento, Mariana B. Oliveira, José R.B. Gomes, João Borges, Benjamin R. Freedman, David J. Mooney, João M.M. Rodrigues, João F. Mano
nossos autores
João M. M. Rodrigues
Investigador Auxiliar
João Mano
Vice-DiretorProjectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
BlueGlue: Bioinspired medical adhesives from marine macroalgae derived biopolymers (BLUEGLUE)
agradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials UIDB/50011/2020 (DOI 10.54499/UIDB/50011/2020), UIDP/50011/2020 (DOI 10.54499/UIDP/50011/2020) &LA/P/0006/2020 (DOI 10.54499/LA/P/0006/2020), financed by national funds through the FCT/MEC (PIDDAC), and within the scope of the project BLUEGLUE (FA_05_2017_031) financed by Fundo Azul (Call – N.° 5/2017) and Direção-Geral de Política do Mar (DGMP) do Ministério Português do Mar. This work was also funded by the European Union's Horizon Europe research and innovation program under the grant agreement No. 101079482 (“SUPRALIFE”). The NMR spectrometers were part of the National NMR Network (PTNMR) and were partially supported by Infrastructure Project N° 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL, and FCT through PIDDAC). M.M.A.S, M.B.O., J.B., and J.M.M.R. gratefully acknowledge FCT for the PhD grant 2020.07156.BD – M.M.A.S. and individual researcher contracts CEECINST/00013/2021 – M.B.O., 2020.00758.CEECIND (DOI 10.54499/2020.00758.CEECIND/CP1589/CT0007) – J.B., CEECIND/01363/2018 (DOI 10.54499/CEECIND/01363/2018/CP1559/CT0022) – J.M.M.R. under the Scientific Employment Stimulus, respectively.