abstract
Graphene and more specifically, nanographene oxide (GO) has been proposed as a highly efficient antitumoral therapy agent. Nevertheless, its cell uptake kinetics, its influence in different types of cells and the possibility of controlling cellular internalization timing, is still a field that remains unexplored. Herein, different cell types have been cultured in vitro for several incubation periods in the presence of 0.075 mg ml(-1) pegylated GO solutions. GO uptake kinetics revealed differences in the agent's uptake amount and speed as a function of the type of cell involved. Osteoblast-like cells GO uptake is higher and faster without resulting in greater cell membrane damage. Moreover, the dependence on the commonly used PEG nature (number of branches) also influences the viability and cell uptake speed. These facts play an important role in the future definition of timing parameters and selective cell uptake control in order to achieve an effective therapy.
keywords
GRAPHENE OXIDE; CARBON NANOTUBES; CANCER-CELLS; NANOPARTICLES; SURFACE; DELIVERY; SIZE; INTERNALIZATION; PARTICLES; THERAPY
subject category
Science & Technology - Other Topics; Materials Science; Physics
authors
Vila, M; Portoles, MT; Marques, PAAP; Feito, MJ; Matesanz, MC; Ramirez-Santillan, C; Goncalves, G; Cruz, SMA; Nieto, A; Vallet-Regi, M
our authors
Sandra Maria Alves da Cruz
PhD StudentGroups
acknowledgements
The work has been financially supported by the Spanish CICYT through project MAT-2008-00736, Spanish National CAM project S2009/MAT-1472 and the Network of Excellence CSO2010-11384-E. M Vila thanks the Spanish Ministry for the RyC grant and the FSE. MC Matesanz thanks the MICINN for the pre-doctoral fellowship. The authors also wish to thank the Electron Microscopy, Confocal Microscopy and Cytometry Center of the Universidad Complutense de Madrid (Spain). P Marques thanks the Ciencia 2007 Program, G Goncalves thanks Iberian Nanotechnology Laboratory (INL) for a PhD grant and S M A Cruz thanks FCT for the PhD grant (SFRH/BD/68598/2010).