abstract
Poly(hydroxybenzene)-trimethoprim conjugates were prepared using methylparaben as substrate of the oxidative enzyme tyrosinase. MALDI-TOF MS analysis showed that the enzymatic oxidation of methylparaben alone leads to the poly(hydroxybenzene) formation. In the presence of trimethoprim, the methylparaben tyrosinase oxidation leads poly(hydroxybenzene)-trimethoprim conjugates. All of these compounds were incorporated into lubricant hydroxyethyl cellulose/glycerol mixtures. Poly(hydroxybenzene)-trimethoprim conjugates were the most effective phenolic structures against the bacterial growth reducing by 96 and 97 % of Escherichia coli and Staphylococcus epidermidis suspensions, respectively (after 24 h). A novel enzymatic strategy to produce antimicrobial poly(hydroxybenzene)-antibiotic conjugates is proposed here for a wide range of applications on the biomedical field.
keywords
CATHETER-RELATED INFECTION; URINARY-TRACT INFECTIONS; AQUEOUS-SOLUTIONS; METHYL PARABEN; ACID PARABENS; HYDROXYETHYLCELLULOSE; PREVENTION; PRODUCTS; HEALTH; DERIVATIVES
subject category
Biotechnology & Applied Microbiology
authors
Goncalves, I; Botelho, CM; Teixeira, A; Abreu, AS; Hilliou, L; Silva, C; Cavaco-Paulo, A
our authors
Idalina José Monteiro Gonçalves
Junior Researcheracknowledgements
The authors Idalina Goncalves and Claudia Botelho would like to acknowledge the NOVO project (FP7-HEALTH-2011.2.3.1- 5) for funding. Loic Hilliou acknowledges the financial support by FCT - Foundation for Science and Technology, Portugal (501100001871), through Grant PEst-C/CTM/LA0025/2013 - Strategic Project - LA 25 - 2013-2014, and by Programa Operacional Regional do Norte (ON.2) through the project