abstract
In this study a novel dinuclear copper(II) complex with adenine and phenanthroline has been synthesized and its structure determined by single crystal X-ray diffraction. In the dinuclear complex [Cu-2(mu-adenine)(2)(phen)(2)(H2O)2](NO3)(4)center dot 0.5H(2)O (phen = 1,10-phenanthroline) (1) the two Cu(II) centres exhibit a distorted square pyramidal coordination geometry linked by two nitrogen donors from adenine bridges leading to a Cu-Cu distance of 3.242(3) angstrom. Intramolecular and intermolecular pi center dot center dot center dot pi interactions as well as an H-bonding network were observed. The antitumor capacity of the complex has been tested in vitro against human cancer cell lines, cervical carcinoma (HeLa) and colorectal adenocarcinoma (Caco-2), by metabolic tests, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide as reagent. The complex 1 has remarkable low IC50 values of 0.87 +/- 0.06 mu M (HeLa) and 0.44 +/- 0.06 mu M (Caco-2), when compared with values for cisplatin against the same cell lines. The interaction of complex 1 with calf thymus DNA (CT DNA) was further investigated by absorption and fluorescence spectroscopic methods. A binding constant of 5.09 x 10(5) M-1 was obtained from UV-vis absorption studies. (C) 2016 Elsevier Inc. All rights reserved.
keywords
PI-PI STACKING; CRYSTAL-STRUCTURE; ANTICANCER ACTIVITY; MAGNETIC-PROPERTIES; NUCLEASE ACTIVITY; CU(II) COMPLEXES; 1,10-PHENANTHROLINE COPPER; PLATINUM COMPLEXES; ANTITUMOR-ACTIVITY; METAL-COMPLEXES
subject category
Biochemistry & Molecular Biology; Chemistry
authors
Ferreira, BJML; Brandao, P; Meireles, M; Martel, F; Correia-Branco, A; Fernandes, DM; Santos, TM; Felix, V
our authors
Bárbara Joana Martins Leite Ferreira
Junior Researcher
Paula Cristina Ferreira da Silva Brandão
Assistant Researcher
Teresa Margarida dos Santos
Assistant Professor
Vítor Félix
Associate Professor with AggregationGroups
1 - inorganic functional nanomaterials and organic-inorganic hybrids
5 - biomedical and biomimetic materials
6 - computer simulation and multiscale modeling
acknowledgements
The authors want to thank University of Aveiro, Institute of materials (CICECO) and the Department of Chemistry for supporting this work. B. J. M. Leite Ferreira thanks Fundacao para a Ciencia e a Tecnologia (FCT) for her postdoctoral grant - Ref. SFRH/BPD/81113/2011.