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authors |
Ferreira, BJML; Brandao, P; Meireles, M; Martel, F; Correia-Branco, A; Fernandes, DM; Santos, TM; Felix, V |
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nationality |
International |
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journal |
JOURNAL OF INORGANIC BIOCHEMISTRY |
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author keywords |
Dinuclear copper(II) complex; Phenanthroline; Adenine; HeLa; Caco-2; DNA-binding |
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keywords |
PI-PI STACKING; CRYSTAL-STRUCTURE; ANTICANCER ACTIVITY; MAGNETIC-PROPERTIES; NUCLEASE ACTIVITY; CU(II) COMPLEXES; 1,10-PHENANTHROLINE COPPER; PLATINUM COMPLEXES; ANTITUMOR-ACTIVITY; METAL-COMPLEXES |
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abstract |
In this study a novel dinuclear copper(II) complex with adenine and phenanthroline has been synthesized and its structure determined by single crystal X-ray diffraction. In the dinuclear complex [Cu-2(mu-adenine)(2)(phen)(2)(H2O)2](NO3)(4)center dot 0.5H(2)O (phen = 1,10-phenanthroline) (1) the two Cu(II) centres exhibit a distorted square pyramidal coordination geometry linked by two nitrogen donors from adenine bridges leading to a Cu-Cu distance of 3.242(3) angstrom. Intramolecular and intermolecular pi center dot center dot center dot pi interactions as well as an H-bonding network were observed. The antitumor capacity of the complex has been tested in vitro against human cancer cell lines, cervical carcinoma (HeLa) and colorectal adenocarcinoma (Caco-2), by metabolic tests, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide as reagent. The complex 1 has remarkable low IC50 values of 0.87 +/- 0.06 mu M (HeLa) and 0.44 +/- 0.06 mu M (Caco-2), when compared with values for cisplatin against the same cell lines. The interaction of complex 1 with calf thymus DNA (CT DNA) was further investigated by absorption and fluorescence spectroscopic methods. A binding constant of 5.09 x 10(5) M-1 was obtained from UV-vis absorption studies. (C) 2016 Elsevier Inc. All rights reserved. |
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publisher |
ELSEVIER SCIENCE INC |
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issn |
0162-0134 |
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year published |
2016 |
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volume |
161 |
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beginning page |
9 |
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ending page |
17 |
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digital object identifier (doi) |
10.1016/j.jinorgbio.2016.04.026 |
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web of science category |
Biochemistry & Molecular Biology; Chemistry, Inorganic & Nuclear |
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subject category |
Biochemistry & Molecular Biology; Chemistry |
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unique article identifier |
WOS:000378977700002
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