Cellulosic Bioethanol from Industrial Eucalyptus globulus Bark Residues Using Kraft Pulping as a Pretreatment


The pulp and paper industry faces an emerging challenge for valorising wastes and side-streams generated according to the biorefinery concept. Eucalyptus globulus bark, an abundant industrial residue in the Portuguese pulp and paper sector, has a high potential to be converted into biobased products instead of being burned. This work aimed to evaluate the ethanol production from E. globulus bark previously submitted to kraft pulping through separate hydrolysis and fermentation (SHF) configuration. Fed-batch enzymatic hydrolysis provided a concentrated hydrolysate with 161.6 g center dot L-1 of cellulosic sugars. S. cerevisiae and Ethanol Red(R) strains demonstrated a very good fermentation performance, despite a negligible xylose consumption. S. passalidarum, a yeast known for its capability to consume pentoses, was studied in a simultaneous co-culture with Ethanol Red(R). However, bioethanol production was not improved. The best fermentation performance was achieved by Ethanol Red(R), which provided a maximum ethanol concentration near 50 g center dot L-1 and fermentation efficiency of 80%. Concluding, kraft pulp from E. globulus bark showed a high potential to be converted into cellulosic bioethanol, being susceptible to implementing an integrated biorefinery on the pulp and paper industrial plants.



subject category

Energy & Fuels


Amandio, MST; Rocha, JMS; Serafim, LS; Xavier, AMRB

our authors


This work was carried out under the Project InPaCTus-Innovative Products and Technologies from Eucalyptus, Project N. 21874 funded by Portugal 2020 through European Regional Development Fund (ERDF) in the frame of COMPETE 2020 no 246/AXIS II/2017. This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES. Authors would also like to thank the CIEPQPF-Strategic Research Centre Project UIDB/00102/2020, funded by the Fundacao para a Ciencia e Tecnologia (FCT).

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