Chemical Characterization of Sambucus nigra L. Flowers Aqueous Extract and Its Biological Implications
authors Ferreira-Santos, P; Badim, H; Salvador, AC; Silvestre, AJD; Santos, SAO; Rocha, SM; Sousa, AM; Pereira, MO; Wilson, CP; Rocha, CMR; Teixeira, JA; Botelho, CM
nationality International
journal BIOMOLECULES
author keywords elderflower; GC-MS; HPLC-MS; chemical profile; phenolic compounds; cytotoxicity; antibacterial activity; antioxidant activity
keywords PHENOLIC-COMPOUNDS; IN-VITRO; ANTIOXIDANT PROPERTIES; MEDICINAL-PLANTS; CHLOROGENIC ACID; URSOLIC ACID; HEPG2 CELLS; RICH SOURCE; QUERCETIN; NARINGENIN
abstract The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 degrees C). The extract obtained at 90 degrees C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was analyzed by GC-MS and HPLC-MS, which allowed the identification of 46 compounds, being quercetin and chlorogenic acid derivatives representative of 86% of the total of phenolic compounds identified in hydrophilic fraction of the aqueous extract. Naringenin (27.2%) was the major compound present in the lipophilic fraction. The antiproliferative effects of the S. nigra extract were evaluated using the colon cancer cell lines RKO, HCT-116, Caco-2 and the extract's antigenotoxic potential was evaluated by the Comet assay in RKO cells. The RKO cells were the most susceptible to S. nigra flower extract (IC50 = 1250 mu g mL(-1)). Moreover, the extract showed antimicrobial activity against Gram-positive bacteria, particularly Staphylococcus aureus and S. epidermidis. These results show that S. nigra based extracts can be an important dietary source of bioactive phenolic compounds that contribute to health-span improving life quality, demonstrating their potential as nutraceutical, functional foods and/or cosmetic components for therapeutic purposes.
publisher MDPI
isbn 2218-273X
year published 2021
volume 11
issue 8
digital object identifier (doi) 10.3390/biom11081222
web of science category 22
subject category Biochemistry & Molecular Biology
unique article identifier WOS:000688963600001
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journal analysis (jcr 2019):
journal impact factor 4.082
5 year journal impact factor Not Available
category normalized journal impact factor percentile 67.172
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