Optimization and modeling of laccase production by Trametes versicolor in a bioreactor using statistical experimental design
authors Tavares, APA; Coelho, MAZ; Agapito, MSM; Coutinho, JAP; Xavier, AMRB
nationality International
journal APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
author keywords bioreactor; enzyme production; kinetic parameters; modeling; optimization; Trametes versicolor
keywords WHITE-ROT FUNGI; SOLID-STATE FERMENTATION; KRAFT PULP; MANGANESE PEROXIDASE; ORGANIC-ACIDS; LIGNIN; DEGRADATION; DECOLORIZATION; TRANSFORMATION; PURIFICATION
abstract Experimental design and response surface methodologies were applied to optimize laccase production by Trametes versicolor in a bioreactor. The effects of three factors, initial glucose concentration (0 and 9 g/L), agitation (100 and 180 rpm), and pH (3.0 and 5.0), were evaluated to identify the significant effects and its interactions in the laccase production. The pH of the medium was found to be the most important factor, followed by initial glucose concentration and the interaction of both factors. Agitation did not seem to play an important role in laccase production, nor did the interaction agitation x medium pH and agitation x initial glucose concentration. Response surface analysis showed that an initial glucose concentration of 11 g/L and pH controlled at 5.2 were the optimal conditions for laccase production by T. versicolor. Under these conditions, the predicted value for laccase activity was > 10,000 U/L, which is in good agreement with the laccase activity obtained experimentally (11,403 U/L). In addition, a mathematical model for the bioprocess was developed. It is shown that it provides a good description of the experimental profile observed, and that it is capable of predicting biomass growth based on secondary process variables.
publisher HUMANA PRESS INC
issn 0273-2289
year published 2006
volume 134
issue 3
beginning page 233
ending page 248
digital object identifier (doi) 10.1385/ABAB:134:3:233
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
unique article identifier WOS:000240216000004
  ciceco authors
  impact metrics
journal analysis (jcr 2019):
journal impact factor 2.277
5 year journal impact factor 2.135
category normalized journal impact factor percentile 36.061
dimensions (citation analysis):
altmetrics (social interaction):



 


Sponsors

1suponsers_list_ciceco.jpg