Analysis of linoleic acid hydroperoxides generated by biomimetic and enzymatic systems through an integrated methodology

abstract

An integrated methodology for the identification and quantification of linoleic acid hydroperoxides (HPODEs) mixtures, obtained by the Fenton's reaction and by enzymatic oxidation, is reported. Unambiguous identification of the HPODEs formed (13-hydroperoxy-(9Z,11E)-octadecadienoic acid, 13-hydroperoxy-(9E,11E)-octadecadienoic acid and 9-hydroperoxyoctadecadienoic acid) was performed by HPLC-MS/MS analysis, while quantification was carried out by HPLC-UV using an external calibration, based on the collection of chromatographic peaks, and measuring of total hydroperoxides by ferrous oxidation-xylenol orange (FOX) method. (C) 2011 Elsevier B.V. All rights reserved.

keywords

MASS-SPECTROMETRY; RENEWABLE RESOURCE; OXIDATION; PRODUCTS; OIL; CHEMISTRY; TISSUES; CHAIN; TIME

subject category

Agriculture

authors

Villaverde, JJ; Santos, SAO; Simoes, MMQ; Neto, CP; Domingues, MRM; Silvestre, AJD

our authors

acknowledgements

FCT and EU are acknowledged for financial support of the ERA-NOEL Project (Novel enzyme tools for production of functional oleochemicals from unsaturated lipids - ERA-IB/BIO/0001/2008), which also funds J.J. Villaverde Postdoctoral Grant.

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