resumo
Image analysis technique was applied to identify morphological changes of pellets from white-rot fungus Trametes versicolor on agitated submerged cultures during the production of exopolysaccharide (EPS) or ligninolytic enzymes. Batch tests with four different experimental conditions were carried out. Two different culture media were used, namely yeast medium or Trametes defined medium and the addition of lignolytic inducers as xylidine or pulp and paper industrial effluent were evaluated. Laccase activity, EPS production, and final biomass contents were determined for batch assays and the pellets morphology was assessed by image analysis techniques. The obtained data allowed establishing the choice of the metabolic pathways according to the experimental conditions, either for laccase enzymatic production in the Trametes defined medium, or for EPS production in the rich Yeast Medium experiments. Furthermore, the image processing and analysis methodology allowed for a better comprehension of the physiological phenomena with respect to the corresponding pellets morphological stages.
palavras-chave
KRAFT PULP; CORIOLUS-VERSICOLOR; SUBMERGED CULTIVATIONS; FILAMENTOUS FUNGI; GROWTH; LIGNIN; MODEL; 1-HYDROXYBENZOTRIAZOLE; POLYSACCHARIDE; MICROORGANISMS
categoria
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
autores
Tavares, APM; Silva, RP; Amaral, AL; Ferreira, EC; Xavier, AMRB
nossos autores
Ana Maria Rebelo Barreto Xavier
Professor Auxiliar
Ana Paula Mora Tavares
Investigador Auxiliar
Rui Pedro Fonseca Ferreira da Silva
Estudante de mestradoGrupos
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
agradecimentos
The authors acknowledge Portucel-Empresa de Celulose e Papel, Cacia, Portugal, SA for the pulp and paper Kraft effluent used in this work. This work was funded by FEDER Funds through the Programa Operacional Factores de Competitividade-COMPETE, and national funds through FCT-Fundacao para a Ciencia e a Tecnologia under the projects PEst-C/CTM/LA/0011/2013 and PEst-C/EQB/LA0020/2013. A. P. M. Tavares acknowledge the financial support of (Programme Ciencia 2008) FCT, Portugal.