resumo
The potential of salicylic acid (SA) encapsulated in porous materials as drug delivery carriers for cancer treatment was studied. Different porous structures, the microporous zeolite NaY, and the mesoporous SBA-15 and MCM-41 were used as hosts for the anti-inflammatory drug. Characterization with different techniques (FTIR, UV/vis, TGA, H-1 NMR, and C-13 CPMAS NMR) demonstrated the successful loading of SA into the porous hosts. The mesoporous structures showed to be very efficient to encapsulate the SA molecule. The obtained drug delivery systems (DDS) accommodated 0.74 mmol (341 mg/g(ZEO)) in NaY and 1.07 mmol (493 mg/g(ZEO)) to 1.23 mmol (566 mg/g(ZEO)) for SBA-15 and MCM-41, respectively. Interactions between SA molecules and pore structures were identified. A fast and unrestricted liberation of SA at 10 min of the dissolution assay was achieved with 29.3, 46.6, and 50.1 mu g/mL of SA from NaY, SBA-15, and MCM-41, respectively, in the in vitro drug release studies (PBS buffer pH 7.4, 37 degrees C). Kinetic modeling was used to determine the release patterns of the DDS. The porous structures and DDS were evaluated on Hs578T and MDA-MB-468 breast cancer cell lines viability. The porous structures are nontoxic to cancer cells. Cell viability reduction was only observed after the release of SA from MCM-41 followed by SBA-15 in both breast cancer cell lines.
palavras-chave
NONSTEROIDAL ANTIINFLAMMATORY DRUGS; RANDOMIZED CONTROLLED-TRIALS; DAILY ASPIRIN; CANCER; SBA-15; RELEASE; RISK; CARCINOMA; PROFILES; SYSTEMS
categoria
Chemistry; Science & Technology - Other Topics; Materials Science
autores
Vilaca, N; Morais-Santos, F; Machado, AF; Sirkecioglu, A; Pereira, MFR; Sardo, M; Rocha, J; Parpot, P; Fonseca, AM; Batazar, F; Neves, IC
nossos autores
João Rocha
Prof. Catedrático
Mariana Coutinho Sardo
Investigador AuxiliarProjectos
Polymorphism in pharmaceuticals: new insights from NMR Crystallography (EXPL/QEQ-QFI/2078/2013)
agradecimentos
F.M.S. and N.V. are recipients of PhD fellowships (SFRH/BD/87139/2012 and SFRH/BD/97797/2013) from Fundacao para a Ciencia e a Tecnologia (FCT, Portugal). M.S. also acknowledges FCT for a postdoc grant (SFRH/BPD/65978/2009). The authors thank the FCT and FEDER-COMPETE-QREN-EU for financial support to the Research Centers, CQ/UM, PEst-C/QUI/UI0686/2013 (F-COMP-01-0124-FEDER-037302), project EXPL/QEQ-QFI/2078/2013 (F-COMP-01-0124-FEDER-041966), Life and Health Sciences Research Institute (ICVS, University of Minho, Portugal), CICECO, PEst-C/CTM/LA0011/2013 (F-COMP-01-0124-FEDER-037271) and LSRE/LCM, PEst-C/EQB/LA0020/2013. The NMR spectrometer is part of the National NMR Network (RNRMN), supported with funds from FCT/QREN.