resumo
Cell surface composition determines all interactions of the cell with its environment, thus cell functions such as adhesion, migration and cell-cell interactions can potentially be controlled by engineering and manipulating the cell membrane. Cell membranes present a rich repertoire of molecules, therefore a versatile ground for modification. However the complex and dynamic nature of the cell surface is also a major challenge for cell surface engineering that should also involve strategies compatible with cell viability. Cell surface engineering by selective chemical reactions or by the introduction of exogenous targeting ligands can be a powerful tool for engineering novel interactions and controlling cell function. In addition to chemical conjugation and modification of functional groups, ligands of interest to modify the surface of cells include recombinant proteins, liposomes or nanoparticles. Here, we review recent efforts to perform changes to cell surface composition. We focus on the engineering of the cell surface with biological, chemical or physical methods to modulate cell functions and control cell-cell and cell-microenvironment interactions. Potential applications of cell surface engineering are also discussed.
palavras-chave
STEM-CELLS; CHEMICAL-MODIFICATION; BIOTIN LIGASE; LIVING CELLS; ADHESION; CHEMISTRY; PROTEINS; DNA; FUNCTIONALIZATION; OLIGONUCLEOTIDES
categoria
Chemistry; Science & Technology - Other Topics; Materials Science
autores
Custodio, CA; Mano, JF
nossos autores
Catarina de Almeida Custódio
Investigador Auxiliar
João Mano
Vice-Diretoragradecimentos
C.A.C. acknowledges funding support from the Portuguese Foundation for Science and Technology (FCT) (fellowship SFRH/BPD/100594/2014). This work was also supported by European Research Council grant agreement ERC-2014-ADG-669858 for project ATLAS.