The influence of Citrate or PEG coating on silver nanoparticle toxicity to a human keratinocyte cell line
authors Bastos, V; de Oliveira, JMPF; Brown, D; Jonhston, H; Malheiro, E; Daniel-da-Silva, AL; Duarte, IF; Santos, C; Oliveira, H
nationality International
journal TOXICOLOGY LETTERS
author keywords Apoptosis; Cell cycle; Citrate-coating; Cytokines; Nanotoxicology; PEG-coating; ROS; Silver nanoparticles
keywords AGGREGATION KINETICS; OXIDATIVE STRESS; EPITHELIAL-CELLS; LIGHT SCATTER; STEM-CELLS; LUNG-CELLS; HUMAN SKIN; CYTOTOXICITY; GENOTOXICITY; SIZE
abstract Surface coating of silver nanoparticles may influence their toxicity, in a way yet to decipher. In this study, human keratinocytes (HaCaT cells) were exposed for 24 and 48 h to well-characterized 30 nm AgNPs coated either with citrate (Cit30 AgNPs) or with poly(ethylene glycol) (PEG30 AgNPs), and assessed for cell viability, reactive oxygen species (ROS), cytokine release, apoptosis and cell cycle dynamics. The results showed that Cit30 AgNPs and PEG30 AgNPs decreased cell proliferation and viability, the former being more cytotoxic. The coating molecules per se were not cytotoxic. Moreover, Ag+ release and ROS production were similar for both AgNP types. Cit30 AgNPs clearly induced apoptotic death, while cells exposed to PEG30 AgNPs appeared to be at an earlier phase of apoptosis, supported by changes in BAX, BCL2 and CASP-3 expressions. Concerning the impact on cell cycle dynamics, both Cit30 and PEG30 AgNPs affected cell cycle regulation of HaCaT cells, but, again, citrate-coating induced more drastic effects, showing earlier downregulation of cyclin B1 gene and cellular arrest at the G2 phase. Overall, this study has shown that the surface coating of AgNPs influences their toxicity by differently regulating cell-cycle and cell death mechanisms. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
publisher ELSEVIER IRELAND LTD
issn 0378-4274
year published 2016
volume 249
beginning page 29
ending page 41
digital object identifier (doi) 10.1016/j.toxlet.2016.03.005
web of science category Toxicology
subject category Toxicology
unique article identifier WOS:000373569800005
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journal impact factor 3.569
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