resumo
Packaging as well as preservation of DNA in nonaqueous media at ambient conditions for long durations is an important research endeavor considering the biomacromolecule as nanoscale substrate for functional biomaterial design and for biotechnological applications. From this perspective, the present work reports both very high concentration dissolution and packaging of DNA in an ionic liquid (IL) without affecting the structural integrity of the biopolymer upon long-term storage. 2-Hydroxyethylammonium formate (2-HEAF), which is an ammonium based IL was able to solubilize 25% w/w of DNA (salmon testes) within 12 h at 25 C. The solubilized DNA in the IL showed long-term chemical and structural stability upon storage under ambient conditions for more than 1 year, which makes the IL a suitable medium for nucleic acid preservation. From isothermal calorimetric (ITC) studies it was evident that the hydrogen bond formed between the IL and DNA was responsible for the high concentration solubility and extended stability, unlike earlier observations for choline based ILs, where both the electrostatic interactions and hydrogen bond were collectively found to be responsible for the phenomenon. Upon auto DNA docking analyses, higher preference for minor-groove over major-groove binding on DNA structure was observed for the IL, and it showed strong ability to promote hydrogen bonding with nucleic acids.
palavras-chave
CHEMICAL-STABILITY; DISSOLUTION; SOLVENTS; EXTRACTION; CHITIN; GROOVE
categoria
Chemistry; Science & Technology - Other Topics; Engineering
autores
Singh, N; Sharma, M; Mondal, D; Pereira, MM; Prasad, K
nossos autores
Dibyendu Mondal
Bolseiro de pós-Doutoramento
Matheus M. Pereira
Investigador
Mukesh Sharma
Bolseiro de pós-Doutoramentoagradecimentos
K.P. thanks CSIR, New Delhi, for the grant of CSIR-Young Scientist Awardees Project and overall financial support. N.S., MS., and D.M. thank UGC and CSIR for NET-JRF and SRF fellowships and AcSIR for PhD registration. Analytical and centralized instrument facility division of the institute is acknowledged for overall analytical support. M.M.P. acknowledges the PhD grant (2740-13-3) and financial support from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Capes. Dr. Vishal Gupta is acknowledged for his help extended during agarose-gel electrophoresis and PCR amplification work. Gopal Bhojani is acknowledged for his help extended during determination of melting point of DNA. This is CSIR-CSMCRI communication No. 167/2016.