resumo
Understanding the mechanisms controlling cell-multilayer film interactions is crucial to the successful engineering of these coatings for biotechnological and biomedical applications. Herein, we present a strategy to tune the cell adhesive properties of multilayers based on marine polysaccharides with and without cross-linking and/or coating with extracellular matrix proteins. Chemical cross-linking of multi layers improved mechanical properties of the coatings but also elicited changes in surface chemistry that alter the adhesion of human umbilical vein endothelial cells. We evaluated a strategy to decouple the mechanical resistant multilayers. Addition of chitosan/alginate multilayers on top of cross-linked films decreased endothelial cell adhesion, spreading, and proliferation to similar levels as uncross-linked films. Our findings highlight the key role of surface chemistry in cell-multilayer film interactions, and these engineered nanocoatings represent a tunable model of cell adhesive and non-adhesive multilayered films. Statement of Significance Multilayered films based on marine-derived polysaccharides were obtained by layer-by-layer (LbL). Biological tests with human umbilical vein endothelial cells (HUVECs) showed the potential of these films to tailor cell adhesion, spreading and proliferation. These multilayered films promise to be versatile and tunable model of cell adhesive and non-adhesive films. (c) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
palavras-chave
POLYELECTROLYTE MULTILAYER FILMS; ATOMIC-FORCE MICROSCOPY; IN-VITRO; BIOMEDICAL APPLICATIONS; REGENERATIVE MEDICINE; SURFACE MODIFICATION; GENIPIN; POLYSACCHARIDES; ADSORPTION; LINKING
categoria
Engineering; Materials Science
autores
Silva, JM; Garcia, JR; Reis, RL; Garcia, AJ; Mano, JF
nossos autores
João Mano
Vice-Diretoragradecimentos
The authors acknowledge the financial support by the Luso-American Foundation and the USA National Institutes of Health (R01 AR062920). Joana M. Silva would also like to acknowledge the Portuguese Foundation for Science and Technology (FCT) for her PhD grant.