Extraction and characterization of collagen from Antarctic and Sub-Antarctic squid and its potential application in hybrid scaffolds for tissue engineering


Collagen is the most abundant protein found in mammals and it exhibits a low immunogenicity, high biocompatibility and biodegradability when compared with others natural polymers. For this reason, it has been explored for the development of biologically instructive biomaterials with applications for tissue substitution and regeneration. Marine origin collagen has been pursued as an alternative to the more common bovine and porcine origins. This study focused on squid (Teuthoidea: Cephalopoda), particularly the Antarctic squid Kondakovia longimana and the Sub -Antarctic squid Illex argentinus as potential collagen sources. In this study, collagen has been isolated from the skins of the squids using acid-based and pepsin-based protocols, with the higher yield being obtained from I. argentinus in the presence of pepsin. The produced collagen has been characterized in terms of physicochemical properties, evidencing an amino acid profile similar to the one of calf collagen, but exhibiting a less preserved structure, with hydrolyzed.portions and a lower melting temperature. Pepsin-soluble collagen isolated from I. argentinus was selected for further evaluation of biomedical potential, exploring its incorporation on poly-e-caprolactone (PC1,) 3D printed scaffolds for the development of hybrid scaffolds for tissue engineering, exhibiting hierarchical features. (C) 2017 Elsevier B.V. All rights reserved.




Materials Science


Coelho, RCG; Marques, ALP; Oliveira, SM; Diogo, GS; Pirraco, RP; Moreira-Silva, J; Xavier, JC; Reis, RL; Silva, TH; Mano, JF

nossos autores


This work was partially funded by ERDF through POCTEP Project 0687_NOVOMAR_1_P and by the European Union Seventh Framework Programme for research, technological development and demonstration under grant agreement on ERC-2012-ADG 20120216-321266 (ComplexiTE). The Portuguese Foundation for Science and Technology (FCT) is also adcnowledged for post-doctoral fellowships of JMS (SFRH/BPD/70230/2010) and RPP (SFRH/BPD/101886/2014), financed by POPH/FSE, and FCT Investigator grant of JX (IF/00616/2013). The authors also want to thank Dr. Julio Maroto (FundaciOn CETMAR, Spain) for the kind offer of the samples of skins of I. argentinus, to Dr. Dario Fassini for the assistance in SDS-PAGE and to Raphael Canadas for assistance in micro-CT data processing.

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