resumo
Good mechanical properties and high injectability are the major requirements to ensure widespread application of calcium phosphate cements (CPCs) as bone substitutes in minimally invasive surgeries. However, obtaining CPCs that exhibit a good compromise between these two properties as well as good biological performance is still a great challenge. This study presents novel solutions to improve these properties, which include (i) co-doping beta-tricalcium phosphate (beta-TCP) powder with Sr and Mn, and (ii) adding small amounts of saccharides (sucrose or fructose) to the setting-liquid solution. The combination of these two strategies enabled full injectability and significantly increased the wet compressive strength of CPCs in comparison to undoped or solely Sr-doped CPCs. Furthermore, the proliferative response of human MG63 osteoblastic cells, their rate of collagen-I secretion, and particularly their growth behaviour on the cement surfaces were also enhanced. The overall improved relevant properties of Mn/Sr co-doped CPCs with added sucrose, including in vitro biological performance, renders them very promising materials for bone regeneration and tissue engineering.
palavras-chave
BETA-TRICALCIUM PHOSPHATE; TO-POWDER RATIO; MECHANICAL-PROPERTIES; IN-VITRO; STRENGTH IMPROVEMENT; HYPODERMIC INJECTION; CALCIUM PHOSPHATES; IONIC MODIFICATION; STRONTIUM; SUBSTITUTION
categoria
Materials Science
autores
Torres, PMC; Marote, A; Cerqueira, AR; Calado, AJ; Abrantes, JCC; Olhero, S; Silva, OABDE; Vieira, SI; Ferreira, JMF
nossos autores
João Carlos de Castro Abrantes
Professor Adjunto
José Maria da Fonte Ferreira
Professor(a) Jubilado(a)
Paula Maria da Costa Torres
Investigador Júnior
Susana Maria Henriques Olhero
Professor AuxiliarGrupos
G3 - Materiais Eletroquímicos, Interfaces e Revestimentos
G5 - Materiais Biomiméticos, Biológicos e Vivos
agradecimentos
This work was developed in the scope of the CICECO-Aveiro Institute of Materials (UID/CTM/50011/2013) and the Institute for Biomedicine iBiMED (UID/BIM/04501/2013) projects, and funded by FEDER funds through the Operational Programme Competitiveness Factors (COMPETE 2020) and the Portuguese Foundation for Science and Technology (FCT). P. M. C. Torres also acknowledges the FCT for the doctoral grant (SFRH/BD/62021/2009). The XRD facility was funded by FEDER funds through QREN-Aviso SAIECT-IEC/2/2010 and Operacao NORTE-07-0162-FEDER-000050. The authors are very thankful to the Hospital Infante D. Pedro of Aveiro, namely to the team involved in sample sterilization, especially to the Chief Nurse Aurea Simoes and to Nurse Margarida Vilarinho.