resumo
Many extracellular matrix proteins have positive influences on the adhesion, proliferation, and differentiation of stem cells into specific cell linages. Fibulin-1 (FBLN1), a member of a growing family of extracellular glycoproteins, contributes to the structure of the extracellular matrix. Here, we investigated the effect of FBLN1 on the ability of human nasal inferior turbinate-derived mesenchymal stem cells (hTMSCs) to undergo osteogenic differentiation. After we generated recombinant FBLN1, the characteristics of FBLN1-treated hTMSCs were evaluated using MTT assay, ALP and mineralization activities, and quantitative real-time PCR. FBLN1 significantly enhanced the adhesion activity (p<0.001) and proliferation of hTMSCs (p<0.05). The ALP and mineralization activities of cells were dramatically increased (p<0.01) after 9 and 12 days of FBLN1 treatment, respectively. This indicated the ability of FBLN1 to induce hTMSCs to differentiate into osteoblasts. Furthermore, increasing the mRNA levels of osteogenic marker genes, such as a transcriptional coactivator with a PDZ-binding motif (TAZ), alkaline phosphatase (ALP), collagen type I (Col I), and osteocalcin (OCN), improved bone repair and regeneration. (C) 2017 Wiley Periodicals, Inc.
palavras-chave
POROUS SILK FIBROIN; CIRCULATORY-SYSTEM; ARTERIAL TREES; FRACTAL MODELS; IN-VIVO; ANGIOGENESIS; VASCULARIZATION; PRINCIPLE; PATTERNS; SCAFFOLD
categoria
Engineering; Materials Science
autores
Pham, LBH; Yoo, YR; Park, SH; Back, SA; Kim, SW; Bjorge, I; Mano, J; Jang, JH
nossos autores
Isabel Bjørge
Estudante de doutoramento
João Mano
Vice-Diretoragradecimentos
Contract grant sponsor: Korea Health Technology R&D Project [Korea Health Industry Development Institute (KHIDI)]; contract grant numbers: HI14C0522, HI14C3228; Contract grant sponsor: National Research Foundation of Korea (NRF); contract grant numbers: NRF-2016R1A2B4008811, NRF-2014R1A2A2A01004325