MoS2 flakes stabilized with DNA/RNA nucleotides: In vitro cell response


Two-dimensional transition metal dichalcogenides (TMDCs), such as MoS2 and WS2, have recently emerged as nanomaterials with potential use in biomedicine. An attractive means to favor their interaction with biological media is the use of proper biomolecules as exfoliating/dispersing agents. Here, MoS2 flakes were stabilized with different small functional biomolecules such as adenosine monophosphate (AMP), guanosine monophosphate (GMP) and flavin mononucleotide (FMN) through the strong nucleotide - MoS2 interaction of Lewis acid-base type, rather than just on the weak dispersive and hydrophobic forces commonly associated with the use of many surfactants. The impact of the nucleotide-stabilized MoS2 flakes on the viability and cell proliferation, on the production of intracellular reactive oxygen species (ROS), and on the preosteoblast differentiation process (early stage) has been also evaluated, as well as the incorporation and intracellular localization of the nanomaterials by MC3T3-E1 and Saos-2 cells. The nucleotide-stabilized MoS2 flakes were found to exhibit excellent biocompatibility. Furthermore, their incorporation did not affect the integrity of the cell plasma membrane, which makes them ideal candidates for delivering drug/gene directly into cells. The in vitro cell response of tumor cells to these nanomaterials differs from that of undifferentiated cells, which provides the basis for their potential use in cancer therapy.




Materials Science


Cicuendez, M; Silva, VS; Santos, J; Coimbra, A; Oliveira, H; Ayan-Varela, M; Paredes, JI; Villar-Rodil, S; Vila, M

nossos autores



M. Cicuendez acknowledges the FCT financial support [Post Doctoral Grant SFRH/BPD/101468/2014] and Operational Program Human Capital (POCH), European Union. V.S. Silva acknowledges financial support from the FCT [SFRH/BPD/110269/2015 Post-Doctoral grant]. H. Oliveira acknowledges financial support FCT SFRH/BPD/111736/2015. M. A.-V., S. V.-R. and J. I. P gratefully acknowledge financial support from the Spanish Ministerio de Economia y Competitividad (MINECO) and the European Regional Development Fund (ERDF) through project MAT2015-69844-R. Partial funding by Plan de Ciencia, Tecnologia e Innovacion (PCTI) 2013-2017 del Principado de Asturias and the ERDF (project IDI/2018/000233) is also acknowledged. M.A-V. is grateful to MINECO for his pre-doctoral contract.

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