Physical Structure and Electrochemical Response of Diamond Graphite Nanoplatelets: From CVD Synthesis to Label-Free Biosensors
authors Santos, NF; Pereira, SO; Fernandes, AJS; Vasconcelos, TL; Fung, CM; Archanjo, BS; Achete, CA; Teixeira, SR; Silva, RF; Costa, FM
nationality International
journal ACS APPLIED MATERIALS & INTERFACES
author keywords nanodiamond; nanographite; charge transfer; impedimetry; label-free; biosensors
keywords CARBON NANOTUBES; ELECTRON-TRANSFER; NITROGEN ADDITION; GRAPHENE; IMMUNOSENSOR; HYDROGEN; GROWTH; PAPER
abstract Hybrid diamond-graphite nanoplatelet (DGNP) thin films are produced and applied to label-free impedimetric biosensors for the first time, using avidin detection as a proof of concept. The DGNPs are synthesized by microwave plasma chemical vapor deposition through H-2/CH4/N-2 gas mixtures in a reproducible and rapid single-step process. The material building unit consists of an inner two-dimensional-like nanodiamond with preferential vertical alignment covered by and covalently bound to nanocrystalline graphite grains, exhibiting {111}(diamond)parallel to{0002}(graphite) epitaxy. The DGNP films morphostructural aspects are of interest for electrochemical transduction, in general, and for Faradaic impedimetric biosensors, in particular, combining enhanced surface area for biorecognition element loading and facile Faradaic charge transfer. Charge transfer rate constants in phosphate buffer saline/[Fe(CN)(6)](4)- solution are shown to increase up to 5.6 x 10(-3) cm s(-1) upon N-2 addition to DGNP synthesis. For the impedimetric detection of avidin, biotin molecules are covalently bound as avidin specific recognition elements on (3-aminopropyl)triethoxysilane-functionalized DGNP surfaces. Avidin quantification is attained within the 10-1000 mu g mL(-1) range following a logarithmic dependency. The limits of detection and of quantitation are 1.3 and 6.4 mu g mL(-1) (19 and 93 nM), respectively, and 2.3 and 13.8 mu g mL(-1) (33 and 200 nM) when considering the nonspecific response of the sensors.
publisher AMER CHEMICAL SOC
issn 1944-8244
isbn 1944-8252
year published 2019
volume 11
issue 8
beginning page 8470
ending page 8482
digital object identifier (doi) 10.1021/acsami.9b00352
web of science category Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
subject category Science & Technology - Other Topics; Materials Science
unique article identifier WOS:000460365300090
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journal impact factor 8.758
5 year journal impact factor 8.901
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