Monitoring plasma protein aggregation during aging using conformation specific antibodies and FTIR spectroscopy
authors Magalhaes, S; Trindade, D; Martins, T; Rosa, IM; Delgadillo, I; Goodfellow, BJ; Silva, OABDE; Henriques, AG; Nunes, A
nationality International
journal CLINICA CHIMICA ACTA
author keywords Protein aggregation; Aging; Conformation specific antibodies; FTIR
keywords TRANSFORM INFRARED-SPECTROSCOPY; SECONDARY STRUCTURES; BIOMARKERS; MECHANISM; TOXICITY; DISEASES
abstract The loss of proteostasis during aging has been well described using different models, however little is known with respect to protein aggregation levels in biofluids with aging. Therefore, the aim of this study was to assess the pattern of age-related protein aggregation in human plasma using two distinct approaches: analysis with conformation-specific antibodies and FTIR spectroscopy. The latter has been widely used in biomedical research to study protein conformational changes in health and disease. Samples from a primary care based-cohort from the Aveiro region, Portugal, were used for slot-blot analyses followed by immunodetection with conformation-specific antibodies and for the acquisition of FTIR spectra. Immunoblot analyses revealed an age-dependent evolution of the protein conformational profile in human plasma, towards a decrease in prefibrillar oligomers and an increase in fibrillar structures. This finding was also supported by PLS-R multivariate analysis of FTIR data, where a positive correlation between the age of the donors and secondary structure of plasma proteins could be observed. Samples from younger donors are characterized by antiparallel beta-sheet-containing structures while intermolecular beta-sheets characterized older samples. Exclusion of age-associated co-morbidities improved the correlation between protein conformational profiles and aging. The results reveal structural changes in human plasma proteins from middle to old age, confirming the age-associated changes in protein aggregation, and support the applicability of FTIR as a reliable approach to study proteostasis during aging.
publisher ELSEVIER
issn 0009-8981
isbn 1873-3492
year published 2020
volume 502
beginning page 25
ending page 33
digital object identifier (doi) 10.1016/j.cca.2019.11.025
web of science category Medical Laboratory Technology
subject category Medical Laboratory Technology
unique article identifier WOS:000512220200004
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journal impact factor 2.615
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