Step-by-step design of proteins for small molecule interaction: A review on recent milestones
authors Pereira, JM; Vieira, M; Santos, SM
nationality International
journal PROTEIN SCIENCE
author keywords computational chemistry; de novo design; protein design; protein synthesis
keywords DIRECT-COUPLING ANALYSIS; COMPUTATIONAL DESIGN; STRUCTURE PREDICTION; RATIONAL DESIGN; ENERGY FUNCTION; PEPTIDE-SYNTHESIS; BINDING; CELL; STABILITY; ELIMINATION
abstract Protein design is the field of synthetic biology that aims at developing de novo custom-made proteins and peptides for specific applications. Despite exploring an ambitious goal, recent computational advances in both hardware and software technologies have paved the way to high-throughput screening and detailed design of novel folds and improved functionalities. Modern advances in the field of protein design for small molecule targeting are described in this review, organized in a step-by-step fashion: from the conception of a new or upgraded active binding site, to scaffold design, sequence optimization, and experimental expression of the custom protein. In each step, contemporary examples are described, and state-of-the-art software is briefly explored.
publisher WILEY
issn 0961-8368
isbn 1469-896X
year published 2021
volume 30
issue 8
beginning page 1502
ending page 1520
digital object identifier (doi) 10.1002/pro.4098
web of science category 19
subject category Biochemistry & Molecular Biology
unique article identifier WOS:000648544600001
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  impact metrics
journal analysis (jcr 2019):
journal impact factor 3.876
5 year journal impact factor 3.23
category normalized journal impact factor percentile 64.815
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