Characterization of levan produced by a Paenibacillus sp. isolated from Brazilian crude oil
authors Mendonca, CMN; Oliveira, RC; Freire, RKB; Piazentin, ACM; Pereira, WA; Gudina, EJ; Evtuguin, DV; Converti, A; Santos, JHPM; Nunes, C; Rodrigues, LR; Oliveira, RPS
nationality International
journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
author keywords Levan; Physicochemical characterization; Thermomechanical stability
keywords ANTIINFLAMMATORY ACTIVITY; FRUCTOOLIGOSACCHARIDES; EXOPOLYSACCHARIDE; POLYSACCHARIDES; POLYMERIZATION; WATER; OLIGOSACCHARIDES; OPTIMIZATION; BACTERIUM; FRUCTANS
abstract A levan-type fructooligosaccharide was produced by a Paenibacillus strain isolated from Brazilian crude oil, the purity of which was 98.5% after precipitation with ethanol and dialysis. Characterization by FTIR, NMR spectroscopy, GC-FID and ESI-MS revealed that it is a mixture of linear beta(2 -+ 6) fructosyl polymers with average degree of polymerization (DP) of 18 and branching ratio of 20. Morphological structure and physicochemical properties were investigated to assess levan microstructure, degradation temperature and thermomechanical features. Thermal Gravimetric Analysis highlighted degradation temperature of 218 degrees C, Differential Scanning Calorimetry (DSC) glass transition at 81.47 degrees C, and Dynamic Mechanical Analysis three frequency-dependent transition peaks. These peaks, corresponding to a first thermomechanical transition event at 86.60 degrees C related to the DSC endothermic event, a second at 170.9 degrees C and a third at 185.2 degrees C, were attributed to different glass transition temperatures of oligo and polyfructans with different DP. Levan showed high morphological versatility and technological potential for the food, nutraceutical, and pharmaceutical industries.
publisher ELSEVIER
issn 0141-8130
isbn 1879-0003
year published 2021
volume 186
beginning page 788
ending page 799
digital object identifier (doi) 10.1016/j.ijbiomac.2021.07.036
web of science category 12
subject category Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science
unique article identifier WOS:000688434300005
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journal impact factor 5.162
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