Inhalable hydrophilic molecule-loaded liposomal dry powder formulations using supercritical CO2-assisted spray-drying
authors Costa, C; Nobre, B; Matos, AS; Silva, AS; Casimiro, T; Corvo, ML; Aguiar-Ricardo, A
nationality International
journal JOURNAL OF CO2 UTILIZATION
author keywords Liposomes; Supercritical carbon dioxide; Quality by design; Pulmonary delivery; Biopharmaceuticals
keywords PHYSICOCHEMICAL CHARACTERIZATION; PULMONARY DELIVERY; STABILITY; DISMUTASE; PROTEINS; ETHANOL; DESIGN; CRYSTALLIZATION; CRYOPROTECTANTS; AEROSOLIZATION
abstract Liposomes are known to be one of the most promising drug delivery systems for carrying and delivering biopharmaceuticals. Yet, both liposomes and biopharmaceuticals are susceptible to destabilization during storage, and thus require cold supply and efficient distribution chains. This drawback can be overcome, though, by converting liposomal suspension into solid form dosage capable of administration via different routes, including the lungs. In this work, we present a synergy between pharmaceutical and supercritical carbon dioxide technologies to assist in liposome drying. Liposomes, encapsulating 5(6)- carboxyfluorescein (CF) as a marker of the internal aqueous phase, were produced and then dried using supercritical CO2 - assisted spray-drying (SASD). CFloaded liposomal dry powder formulations were thus obtained. After resuspension in water to remove the trehalose, the liposomes maintained their structure and the CF encapsulation efficiency remained above 95 %. To optimize the process, a quality-by-design approach using the design of experiments tool was used. Then, the powders were submitted to storage stability assays at relative humidity of 4 %, 50 % and 78 % for 30 days. Results showed that the dry powder formulations were able to maintain liposome stability at relative humidity of 4 % and 50 % at 20 degrees C for 30 days.
publisher ELSEVIER SCI LTD
issn 2212-9820
isbn 2212-9839
year published 2021
volume 53
digital object identifier (doi) 10.1016/j.jcou.2021.101709
web of science category 12
subject category Chemistry, Multidisciplinary; Engineering, Chemical
unique article identifier WOS:000701977600005
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journal analysis (jcr 2019):
journal impact factor 5.993
5 year journal impact factor 6.193
category normalized journal impact factor percentile 85.534
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