Chronic exercise training attenuates prostate cancer-induced molecular remodelling in the testis
authors Matos, B; Patricio, D; Henriques, MC; Freitas, MJ; Vitorino, R; Duarte, IF; Howl, J; Oliveira, PA; Seixas, F; Duarte, JA; Ferreira, R; Fardilha, M
nationality International
journal CELLULAR ONCOLOGY
author keywords Prostate cancer; Exercise training; Testicular function; Oxidative stress; DNA repair; Metabolism
keywords RECREATIONAL PHYSICAL-ACTIVITY; DNA-DAMAGE; RISK-FACTOR; STEROIDOGENIC DISORDERS; SEMEN QUALITY; RAT TESTIS; TESTOSTERONE; MEN; YOUNG; SPERMATOGENESIS
abstract Purpose Prostate cancer is a major cause of cancer-related death in males worldwide and, in addition to impairing prostate function, also causes testicular adaptations. In this study, we aim to investigate the preventive effect of exercise training on PCa-induced testicular dysfunction. Methods As a model, we used fifty Wistar Unilever male rats, randomly divided in four experimental groups. Prostate cancer was chemically and hormonally induced in two groups of animals (PCa groups). One control group and one PCa group was submitted to moderate intensity treadmill exercise training. Fifty weeks after the start of the training the animals were sacrificed and sperm, prostate, testis and serum were collected and analyzed. Sperm concentration and morphology, and testosterone serum levels were determined. In addition, histological analyses of the testes were performed, and testis proteomes and metabolomes were characterized. Results We found that prostate cancer negatively affected testicular function, manifested as an arrest of spermatogenesis. Oxidative stress-induced DNA damage, arising from reduced testis blood flow, may also contribute to apoptosis of germ cells and consequential spermatogenic impairment. Decreased utilization of the glycolytic pathway, increased metabolism of ketone bodies and the accumulation of branched chain amino acids were also evident in the PCa animals. Conversely, we found that the treadmill training regimen activated DNA repair mechanisms and counteracted several metabolic alterations caused by PCa without impact on oxidative stress. Conclusions These findings confirm a negative impact of prostate cancer on testis function and suggest a beneficial role for exercise training in the prevention of prostate cancer-induced testis dysfunction.
publisher SPRINGER
issn 2211-3428
isbn 2211-3436
year published 2021
volume 44
issue 2
beginning page 311
ending page 327
digital object identifier (doi) 10.1007/s13402-020-00567-9
web of science category 17
subject category Oncology; Cell Biology; Pathology
unique article identifier WOS:000579689300002
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journal impact factor 5.304
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