resumo
Mesoporous silica nanoparticles (MSNPs) have attracted much attention in many biomedical applications. One of the fields in which smart functional nanosystems have found wide application is cancer treatment. Here, we present new silica nanopartide-based systems which have been explored as efficient vehicles to transport and deliver photosensitizers (PSs) into tumor tissues during photodynamic therapy (PDT). In this work, we report the preparation, characterization, and in vitro studies of distinct shaped MSNPs grafted with S-glycoside porphyrins (Pors). The ensuing nanomaterials were fully characterized, and their properties as third-generation PSs for PDT against two bladder cancer cell Lines, HT-1376 and UM-UC-3, were examined. The best uptake results were obtained for MSNP-PS2, while MSNP-PS1 showed the lowest cellular uptake among the nanocarriers tested, but revealed the best phototoxicity in both cancer cells. Overall, the phototoxicity was higher with MSNPs than with mesoporous silica nanorods (MSNRs) and higher uptake and phototoxicity were consistently observed in UM-UC-3 rather than in HT-1376 cancer cells.
palavras-chave
DRUG-DELIVERY; PHOTOPHYSICAL PROPERTIES; PORPHYRIN; CELL; PHOTOCYTOTOXICITY; MECHANISM; MCM-41
categoria
Materials Science
autores
Borzecka, W; Pereira, PMR; Fernandes, R; Trindade, T; Torres, T; Tome, JPC
nossos autores
Tito Trindade
Professor Catedrático
Wioleta Anna Borzecka
Estudante de doutoramentoagradecimentos
Thanks are due to FCT/MEC for the financial support to LAQV-REQUIMTE (UIDB/50006/2020), CICECO-Aveiro Institute of Materials (UIDB/50011/2020), CIBB (UIDB/04539/2020 and UIDP/04539/2020) and CQE (UIDB/00100/2020) research units, through national funds and where applicable cofinanced by the FEDER, within the PT2020 Partnership Agreement. The work was also supported by the Spanish MINECO (PID2020-116490GB-I00, Porphyrinoids), MINECO/ERA-NET (PCIN-2017-042/EuroNanoMed2017-191, TEMPEAT), and Portuguese COMPETE (POCI-01-0145-FEDER-007440). IMDEA Nanociencia also acknowledges support from the Severo Ochoa'' Programme for Centres of Excellence in R&D (MINECO, Grant SEV-2016-0686). We further wish to thank the Seventh Framework Programme (FP7-People-2012-ITN) for funding the SO2S project (grant agreement number: 316975). The authors also acknowledge FCT for the doctoral research fellowship SFRH/BD/85941/2012 (to PMRP).