resumo
Garcinia mangostana L., also known as the mangosteen tree, is a native medicinal plant in Southeast Asia having a wide variety of pharmacologically active compounds, including xanthonoid mangostin. In this study, we examined the pharmacological activities of the selected semi-synthetic mangostin derivative, namely, amoebicidal activity, encystation inhibition, excystation activity, and removal capacity of adhesive Acanthamoeba from the surface of contact lens (CL). Among the three derivatives, C1 exhibited promising anti-Acanthamoeba activity against Acanthamoeba triangularis WU19001 trophozoites and cysts. SEM images displayed morphological changes in Acanthamoeba trophozoites, including the loss of acanthopodia, pore formation in the cell membrane, and membrane damage. In addition, the treated cyst was shrunken and adopted an irregular flat cyst shape. Under a fluorescence microscope, acridine orange and propidium iodide (AO/PI) staining revealed C1 induced condensation of cytoplasm and chromatin with the loss of cell volume in the treated trophozoites, while calcofluor white staining demonstrated the leakage of cell wall in treated cysts, leading to cell death. Interestingly, at the concentration ranges in which C1 showed the anti-Acanthamoeba effects (IC50 values ranging from 0.035-0.056 mg/mL), they were not toxic to Vero cells. C1 displayed the highest inhibitory effect on A. triangularis encystation at 1/16xMIC value (0.004 mg/mL). While C1 demonstrated the excystation activity at 1/128xMIC value with a high rate of 89.47%. Furthermore, C1 exhibited the removal capacity of adhesive Acanthamoeba from the surface of CL comparable with commercial multipurpose solutions (MPSs). Based on the results obtained, C1 may be a promising lead agent to develop a therapeutic for the treatment of Acanthamoeba infections and disinfectant solutions for CL.
palavras-chave
PRENYLATED XANTHONES; THERAPEUTIC AGENTS; CHLORHEXIDINE; INFECTIONS; DIAGNOSIS; KERATITIS; TROPHOZOITES; DISEASE; CYSTS; EYE
categoria
Science & Technology - Other Topics
autores
Chuprom, J; Sangkanu, S; Mitsuwan, W; Boonhok, R; Mahabusarakam, W; Singh, LR; Dumkliang, E; Jitrangsri, K; Paul, AK; Surinkaew, S; Wilairatana, P; Pereira, MD; Rahmatullah, M; Wiart, C; Oliveira, SMR; Nissapatorn, V
nossos autores
Maria de Lourdes Gomes Pereira
Professor Associado com AgregaçãoProjectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This work was supported by the the Royal Patronage of Her Royal Highness Princess Maha Chakri Sirindhorn-Botanical Garden of Walailak University, Nakhon Si Thammarat, under the project entitled: Medicinal Thai Native Plants against Acanthamoeba triangularis as a serious eye infection (WUBG 031-2565) and project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, FCT/MEC (PIDDAC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.