Biomimetic Graphene/Spongin Scaffolds for Improved Osteoblasts Bioactivity via Dynamic Mechanical Stimulation


Biomimetics offers excellent prospects for design a novel generation of improved biomaterials. Here the controlled integration of graphene oxide (GO) derivatives with a 3D marine spongin (MS) network is explored to nanoengineer novel smart bio-based constructs for bone tissue engineering. The results point out that 3D MS surfaces can be homogeneously coated by layer-by-layer (LbL) assembly of oppositely charged polyethyleneimine (PEI) and GO. Notably, the GOPEI@MS bionanocomposites present a high structural and mechanical stability under compression tests in wet conditions (shape memory). Dynamic mechanically (2 h of sinusoidal compression cyclic interval (0.5 Hz, 0-10% strain)/14 d) stimulates GOPEI@MS seeded with osteoblast (MC3T3-E1), shows a significant improvement in bioactivity, with cell proliferation being two times higher than under static conditions. Besides, the dynamic assays show that GOPEI@MS bionanocomposites are able to act as mechanical stimulus-responsive scaffolds able to resemble physiological bone extracellular matrix (ECM) requirements by strongly triggering mineralization of the bone matrix. These results prove that the environment created by the system cell-GOPEI@MS is suitable for controlling the mechanisms regulating mechanical stimulation-induced cell proliferation for potential in vivo experimentation.




Biochemistry & Molecular Biology; Materials Science; Polymer Science


Semitela, A; Carvalho, S; Fernandes, C; Pinto, S; Fateixa, S; Nogueira, HIS; Bdikin, I; Completo, A; Marques, PAAP; Goncalves, G

nossos autores


G.G., a.S., I.B. and S.F. thanks to the FundacAo para a Ciencia e Tecnologia (FCT) for the Programme Stimulus of Scientific Employment - Individual Support (CEECIND/01913/2017), PhD grant SFRH/BD/133129/2017, IF/00582/2015 and research contract REF-069-88-ARH-2018, respectively. The financial support of TEMA by the projects UIDB/00481/2020 and UIDP/00481/2020 from FCT; and CENTRO-01-0145-FEDER-022083 - Centro Portugal Regional Operational Programme (Centro 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund. CICECO-Aveiro Institute of Materials, University of Aveiro, grant FCT/MEC: UIDB/50011/2020 & UIDP/50011/2020 for Confocal Raman microscopy characterization.

Partilhe este projeto

Publicações similares

Usamos cookies para atividades de marketing e para lhe oferecer uma melhor experiência de navegação. Ao clicar em “Aceitar Cookies” você concorda com nossa política de cookies. Leia sobre como usamos cookies clicando em "Política de Privacidade e Cookies".