resumo
Biomimetics offers excellent prospects for design a novel generation of improved biomaterials. Here the controlled integration of graphene oxide (GO) derivatives with a 3D marine spongin (MS) network is explored to nanoengineer novel smart bio-based constructs for bone tissue engineering. The results point out that 3D MS surfaces can be homogeneously coated by layer-by-layer (LbL) assembly of oppositely charged polyethyleneimine (PEI) and GO. Notably, the GOPEI@MS bionanocomposites present a high structural and mechanical stability under compression tests in wet conditions (shape memory). Dynamic mechanically (2 h of sinusoidal compression cyclic interval (0.5 Hz, 0-10% strain)/14 d) stimulates GOPEI@MS seeded with osteoblast (MC3T3-E1), shows a significant improvement in bioactivity, with cell proliferation being two times higher than under static conditions. Besides, the dynamic assays show that GOPEI@MS bionanocomposites are able to act as mechanical stimulus-responsive scaffolds able to resemble physiological bone extracellular matrix (ECM) requirements by strongly triggering mineralization of the bone matrix. These results prove that the environment created by the system cell-GOPEI@MS is suitable for controlling the mechanisms regulating mechanical stimulation-induced cell proliferation for potential in vivo experimentation.
palavras-chave
DRIED COLLAGEN SCAFFOLDS; IN-VITRO; PROLIFERATION; BIOMATERIALS; NANOSHEETS; GROWTH
categoria
Biochemistry & Molecular Biology; Materials Science; Polymer Science
autores
Semitela, A; Carvalho, S; Fernandes, C; Pinto, S; Fateixa, S; Nogueira, HIS; Bdikin, I; Completo, A; Marques, PAAP; Goncalves, G
nossos autores
Helena Isabel Seguro Nogueira
Professor Auxiliar com Agregação
Sara Fateixa
Investigador Júnior
Susana Cristina dos Santos Pinto
Investigador JúniorProjectos
Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)
agradecimentos
G.G., a.S., I.B. and S.F. thanks to the FundacAo para a Ciencia e Tecnologia (FCT) for the Programme Stimulus of Scientific Employment - Individual Support (CEECIND/01913/2017), PhD grant SFRH/BD/133129/2017, IF/00582/2015 and research contract REF-069-88-ARH-2018, respectively. The financial support of TEMA by the projects UIDB/00481/2020 and UIDP/00481/2020 from FCT; and CENTRO-01-0145-FEDER-022083 - Centro Portugal Regional Operational Programme (Centro 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund. CICECO-Aveiro Institute of Materials, University of Aveiro, grant FCT/MEC: UIDB/50011/2020 & UIDP/50011/2020 for Confocal Raman microscopy characterization.