Synthesis, characterization, cytotoxicity effect and DNA cleavage study of symmetric dinuclear chloro and azido bridged copper(II) complexes of napthyl-pyrazole based ligand
authors Jana, A; Brandao, P; Mondal, G; Bera, P; Santra, A; Jana, AD; Mokhamatam, RB; Manna, SK; Bhattacharyya, N; Bera, P
nationality International
journal INORGANICA CHIMICA ACTA
author keywords Bridged dinuclear; Copper(II) complexes; Napthyl pyrazole; DNA cleavage; Cytotoxicity
keywords CRYSTAL-STRUCTURE DETERMINATION; NITROGEN SINGLE BOND; STRAND SCISSION; RUTHENIUM COMPLEXES; ANTICANCER ACTIVITY; CU(II) COMPLEXES; CELL-DEATH; IN-VITRO; AGENTS; DERIVATIVES
abstract Symmetric dinuclear chloro copper(II) complex [Cu(L)(Cl)(mu-Cl)](2) (1) and azo dinuclear azido copper(II) complex [Cu-2(L)(2)(N-3)(3)(mu(2)-N-3)](n) (2) [where L represents (5-methyl-pyrazol-1-ylmethyl)-napthalen-1-ylmethyl-amine] have been synthesized to examine the effect of napthyl group in the structure of pyrazole based dinuclear copper(II) complexes in DNA nuclease activity. The structure of 1 and 2 are characterized by X-ray crystallography, electrochemistry and various spectroscopic techniques. Coordinating ligand L is generated in situ from bis(3,5-dimethyl-pyrazol-1-ylmethyl)-napthalen-1-ylmethyl-amine (A) during complexation. Cytotoxic potential of free ligand (A), synthesized complexes 1, 2 and one cobalt(II) complex derived from ligand A, Co-II(A)Cl-2 (3) are analyzed using MTT cytotoxicity assay in U937 human monocytic cell line. Complexes 1 and 2 show very potent cytotoxicity (IC50 = 13-17 mu M); the best IC50 value is found for 1. LDH assay revealed that A and 3 has greater necrotic activity than the copper complexes. However, the results of DNA cleavage study clearly demonstrated that symmetric bridged dinuclear complexes with napthyl group lead to high level of nuclease activity 72-75% in the presence of glutathione. The bridged dinuclear copper(II) complexes undergo facile transformation to Cu(I) centre through inner sphere electron transfer mechanism (ISET) in presence of glutathione which facilitate the formation of free radicals/ions for DNA cleavage. Lacking of any reducible metal center in mononuclear cobalt(II) complex make it inactive towards free radicals generation in DNA cleavage activity.
publisher ELSEVIER SCIENCE SA
issn 0020-1693
year published 2018
volume 482
beginning page 621
ending page 634
digital object identifier (doi) 10.1016/j.ica.2018.06.054
web of science category Chemistry, Inorganic & Nuclear
subject category Chemistry
unique article identifier WOS:000443282000080
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