resumo
Recent advances on brewer's yeast cell wall polysaccharides have unraveled exquisite structural features and diverse composition with (81-*3), (81-*6), (a1-*4), (81-*4)-mix-linked glucans that are recognized to interact with different cell receptors and trigger specific biological responses. Herein, a comprehensive showcase of structure-biofunctional relationships between yeast polysaccharides and their biological targets is highlighted, with a focus on polysaccharide features that govern the biomedical activity. The insolubility of 8-glucans is a crucial factor for binding and activation of Dectin-1 receptor, operating as adjuvants of immune responses. Contrarily, soluble low molecular weight 8-glucans have a strong inhibition of reactive oxygen species production, acting as antagonists of Dectin-1 mediated signaling. Soluble glucan-protein moieties can also act as antitumoral agents. The balance between mannoproteins-TLR2 and 8-glucans-Dectin-1 receptors-activation is crucial for osteogenesis. Biomedical applications value can also be obtained from yeast microcapsules as oral delivery systems, where highly branched (81-*6)-glucans lead to higher receptor affinity.
palavras-chave
ANTITUMOR IMMUNE-RESPONSES; BETA-GLUCAN PARTICLES; LAGER BREWING YEAST; CELL-WALL; SACCHAROMYCES-CEREVISIAE; TARGETED DELIVERY; PARTICULATE; DIFFERENTIATION; NANOPARTICLES; 3)-BETA-D-GLUCAN
categoria
Chemistry; Polymer Science
autores
Bastos, R; Oliveira, PG; Gaspar, VM; Mano, JF; Coimbra, MA; Coelho, E
nossos autores
Elisabete Verde Martins Coelho
Bolseiro de pós-Doutoramento
João Mano
Vice-Diretor
Vítor Gaspar
Professor Auxiliaragradecimentos
TThe authors acknowledge to FCT/MEC for the financial support of the project Yeast4FoodMed (POCI-01-045-FEDER-030936), LAQV/REQUIMTE (UIDB/50006/2020, UIDP/50006/2020), and CICECO (UIDB/50011/2020, UIDP/50011/2020) through national funds and, where applicable, co-financed by the FEDER -Fundo Europeu de Desenvolvimento Regional, within the PT2020 Partnership Agreement. Rita Bastos was supported with individual grant by FCT (PD/BD/114579/2016), Elisabete Coelho thanks the research contract (CDLCTTRI-88-ARH/2018 -REF. 049-88-ARH/2018) funded by national funds (OE), through FCT, in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.