resumo
Biopolymeric implantable patches are popular scaffolds for myocardial regeneration applications. Besides being biocompatible, they can be tailored to have required properties and functionalities for this application. Recently, fibrillar biobased nanostructures prove to be valuable in the development of functional biomaterials for tissue regeneration applications. Here, periodate-oxidized nanofibrillated cellulose (OxNFC) is blended with lysozyme amyloid nanofibrils (LNFs) to prepare a self-crosslinkable patch for myocardial implantation. The OxNFC:LNFs patch shows superior wet mechanical properties (60 MPa for Young's modulus and 1.5 MPa for tensile stress at tensile strength), antioxidant activity (70% scavenging activity under 24 h), and bioresorbability ratio (80% under 91 days), when compared to the patches composed solely of NFC or OxNFC. These improvements are achieved while preserving the morphology, required thermal stability for sterilization, and biocompatibility toward rat cardiomyoblast cells. Additionally, both OxNFC and OxNFC:LNFs patches reveal the ability to act as efficient vehicles to deliver spermine modified acetalated dextran nanoparticles, loaded with small interfering RNA, with 80% of delivery after 5 days. This study highlights the value of simply blending OxNFC and LNFs, synergistically combining their key properties and functionalities, resulting in a biopolymeric patch that comprises valuable characteristics for myocardial regeneration applications.
autores
Tiago Carvalho, Raquel Bártolo, Alexandra Correia, Carla Vilela, Shiqi Wang, Hélder A. Santos, Carmen S. R. Freire
nossos autores
Carla Vilela
Professor Auxiliar
Carmen Sofia da Rocha Freire Barros
Investigador CoordenadorProjectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This work was developed within the scope of the project CICECO–Aveiro Institute of Materials, UIDB/50011/2020 (DOI 10.54499/UIDB/50011/2020), UIDP/50011/2020 (DOI 10.54499/UIDP/50011/2020), and LA/P/0006/2020 (DOI 10.54499/LA/P/0006/2020), financed by national funds through the FCT/MEC (PIDDAC). The Portuguese Foundation for Science and Technology (FCT) is also acknowledged for the doctoral grant to T.C. (SFRH/BD/130458/2017 and COVID/BD/152394/2022), and the research contracts under Scientific Employment Stimulus to C.V. (DOI 10.54499/2021.01571.CEECIND/CP1659/CT0024) and C.S.R.F. (DOI 10.54499/CEECIND/00464/2017/CP1459/CT033. S.W. acknowledges the Research Council of Finland (Grant No. 331106). H.A.S. acknowledges the Research Council Finland (Grant No. 331151) and UMCG Research Funds for financial support. The authors also acknowledge the Electron Microscopy Unit of the University of Helsinki for providing the facilities for SEM imaging, funded by Biocenter Finland.