resumo
Despite recent years' remarkable progress in medical procedures for bone reconstruction, the implementation of newly developed biomedical materials is still facing technical drawbacks, such as, lack of mechanical strength, impaired cellular proliferation and differentiation, limited osteogenesis and issues related to bone infection, osseointegration and vascularization in large defects.This paper presents a methodology to predict the potential behaviour of bioceramic-based composite scaffolds for bone engineering applications, based on the evaluation of its morphological features, mechanical properties, and mechanical stimulus' input to cells. Chitosan/biphasic calcium phosphates (CH/BCP) composite scaffolds with different structural parameters, namely pore size, filament thickness and layer overlapping, pore size gradient and irregularity on the filament positioning were considered. The finite element simulation was vali-dated against experimental results on 3D printed scaffolds, being proposed an in silico model for the charac-terization of the scaffolds' material composition. With this study, the influence of the CH/BCP bulk material mechanical properties on the scaffolds mechanical behaviour was demonstrated. CH/BCP show to behave ac-cording to a Yeoh hyperelastic material model. Scaffolds with a regular structure and 500 mu m filament distance (PL500) or irregular architecture models (IS2 scaffold) demonstrated better equilibrium among the analysed features, namely to promote cell adhesion and migration, nutrient and oxygen flow, vascularization, mechanical capacity to support the load without experiencing premature failure and initial bone formation through the transmission of bone growth mechanical stimulus. A good correlation between this study and literature data was obtained, which highlighted the suitability of the proposed methodology to infer on the scaffold biomechanical behaviour and allowing the scaffold improvement at an early stage of conceptualization.
palavras-chave
CANCELLOUS BONE
categoria
Engineering
autores
Rosa, N; Pouca, MV; Torres, PMC; Olhero, SM; Jorge, RN; Parente, M
nossos autores
Natacha Alexandra Branco Rosa
Investigador
Paula Maria da Costa Torres
Investigador Auxiliar
Susana Maria Henriques Olhero
Professor AuxiliarProjectos
Human Platelet Lysates-based Scaffolds for Interfacial Multi-tissue Repair (INTERLYNK)
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This study was funded by European Union's Horizon 2020 research and innovation programme under grant agreement No 953169 under the scope of InterLynk project. We acknowledge the support from the Foundation for Science and Technology (FCT), Lisbon, Portugal, under Project LAETA-UIDB/50022/2020. The work is also funded by FEDER through the COMPETE 2020 Programme and National Funds under the project 2BBone (PTDC/CTM-CER/29940/2017) . The project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MCTES (PIDDAC) is also acknowledged. P. M. C. Torres and S M Olhero acknowledge FCT for CEECIND/01891/2017 and CEECIND/03393/2017 contracts, respectively.