resumo
In the present study, H-1 HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed.
palavras-chave
DOSE 2 GY; BREAST-CANCER; 3-DIMENSIONAL SPHEROIDS; IONIZING-RADIATION; MOBILE LIPIDS; K562 CELLS; IN-VITRO; H-1-NMR; SPECTROSCOPY; APOPTOSIS
categoria
Biochemistry & Molecular Biology
autores
Duarte, IF; Lamego, I; Marques, J; Marques, MPM; Blaise, BJ; Gil, AM
nossos autores
Ana Maria Pissarra Coelho Gil
Professor Associado com Agregação
Iola Melissa Fernandes Duarte
Investigador Principalagradecimentos
Funding is acknowledged from Fundacao para a Ciencia e Tecnologia (FCT) in Portugal (FCT/PTDC/SAU-BEB-66896/2006 and FCT/SFRH/BD/63916/2009), the Associate Laboratory IBMC-INEB (for kindly providing the MG-63 cell line) and CRMN- Centre de RMN a Tres Hauts Champs, CNRS/ENS Lyon, France, for access to the 700 MHz NMR spectrometer. B.J.B. acknowledges support from Bruker Biospin, the French National Institute for Cancer INCa (Grant INCa-2008-060) and the CNRS. Financial support by the Access to Research Infrastructures activity in the sixth Framework Programme of the EC (Contract # RII3-026145, EU-NMR) for conducting the research is gratefully acknowledged.