resumo
Biohybrids consisting of gelatin (G) and calcium phosphate silicate/wollastonite (CPS/W) have not been prepared so far. In this work our results are focused on the possibility of obtaining G-CPS/W bioactive hybrids in vitro. XRD, FTIR, SEM/EDS techniques were employed to characterize the synthesized hybrid materials. FTIR shows that before immersion in 1.5 SBF the "red shift" of COO-band for pure G is observed. The presence of this bond could be attributed to the formation of COO-Ca2+ via non-biomimetic route. After immersion in 1.5 SBF, FTIR shows the presence of A-and B-type carbonate containing hydroxyapatite (A/B-CO(3)HA). ESD and FTIR show that small amount of calcite (CaCO3) are present after in vitro test in 1.5 SBF for 3 days. XRD reveals that CO3 HA and small amounts of CaCO3 can be detected after in vitro test. SEM results obtained for immersed samples show that hydroxyapatite (HA) particles fully covered the surface of the hybrids by a layer composed of spherulites. At higher magnification, very small elongated crystallites could be observed.
palavras-chave
SIMULATED BODY-FLUID; HYDROXYAPATITE/GELATIN NANOCOMPOSITE; CARBONATED HYDROXYAPATITE; GLASS-CERAMICS; CROSS-LINKAGE; COLLAGEN; CRYSTALS; APATITE; MINERALIZATION; NUCLEATION
categoria
Chemistry
autores
Radev, L; Hristov, V; Fernandes, MHV; Salvado, IMM
nossos autores
Isabel Margarida Miranda Salvado
Professor associado
Maria Helena Figueira Vaz Fernandes
Professor(a) Jubilado(a)agradecimentos
The financial support of the Bulgarian NSF under contract. VU-TN-102/2005 is gratefully acknowledged.