Characterization of Phenolic Components in Polar Extracts of Eucalyptus globulus Labill. Bark by High-Performance Liquid Chromatography-Mass Spectrometry

authors	Santos, SAO; Freire, CSR; Domingues, MRM; Silvestre, AJD; Neto, CP
nationality	International
journal	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
author keywords	Eucalyptus globulus Labill.; bark; phenolic compounds; flavonoids; HPLC-ESI-MS; tandem mass spectrometry
keywords	MOLECULAR-WEIGHT POLYPHENOLS; E-RUDIS; HYDROLYZABLE TANNINS; STEM BARK; ACID; CAMALDULENSIS; FLAVONOL; LEAVES; WOOD; AGLYCONES
abstract	High-performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (MS") were used to investigate the phenolic constituents in methanol, water, and methanol/water extracts of Eucalyptus globulus Labill. bark. Twenty-nine phenolic compounds were identified, 16 of them referenced for the first time as constituents of E. globulus bark, namely, quinic, dihydroxyphenylacetic, and caffeic acids, bis(hexahydroxydiphenoyl (HHDP))-glucose, galloyl-bis(HHDP)-glucose, galloyl-HHDP-glucose, isorhamentin-hexoside, quercetin-hexoside, methylellagic acid (EA) pentose conjugate, myricetin-rhamnoside, isorhamnetin-rhamnoside, mearnsetin, phloridzin, mearnsetin-hexoside, luteolin, and a proanthocyanidin B-type dimer. Digalloylglucose was identified as the major compound in the methanol and methanol/water extracts, followed by isorhamnetin-rhamnoside in the methanol extract and by catechin in the methanol/water extract, whereas in the water extract catechin and galloyl- HHDP-glucose were identified as the predominant components. The methanol/water extract was shown be the most efficient to isolate phenolic compounds identified in E. globulus bark.
publisher	AMER CHEMICAL SOC
issn	0021-8561
year published	2011
volume	59
issue	17
beginning page	9386
ending page	9393
digital object identifier (doi)	10.1021/jf201801q
web of science category	Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology
subject category	Agriculture; Chemistry; Food Science & Technology
unique article identifier	WOS:000294585100043