resumo
Two different structures of zeolites, faujasite (FAU) and Linde type A (LTA), were studied to investigate their suitability for drug delivery systems (DDS). The zeolites in the sodium form (NaY and NaA) were used as hosts for encapsulation of alpha-cyano-4-hydroxycinnamic acid (CHC). CHC, an experimental anticancer drug, was encapsulated in both zeolites by diffusion in liquid phase. These new drug delivery systems, CHC@zeolite, were characterized by spectroscopic techniques (FTIR, H-1 NMR, C-13 and Al-27 solid-state MAS NMR, and UV-vis), chemical analysis, powder X-ray diffraction (XRD) and scanning electron microscopy (SEM). The effect of the zeolites and CHC@zeolite drug deliveries on HCT-15 human colon carcinoma cell line viability was evaluated. Both zeolites alone revealed no toxicity to HCT-15 cancer cells. Importantly, CHC@zeolite exhibit an inhibition of cell viability up to 585-fold, when compared to the non-encapsulated drug. These results indicate the potential of the zeolites for drug loading and delivery into cancer cells to induce cell death.
palavras-chave
SULFONAMIDE ANTIBIOTICS; SYNTHETIC ZEOLITES; HUMAN-MELANOMA; HOST-GUEST; Y ZEOLITE; IN-VITRO; CANCER; NANOPARTICLES; RELEASE; CELLS
categoria
Chemistry; Science & Technology - Other Topics; Materials Science
autores
Amorim, R; Vilaca, N; Martinho, O; Reis, RM; Sardo, M; Rocha, J; Fonseca, AM; Baltazar, F; Neves, IC
nossos autores
João Rocha
Prof. Catedrático
Mariana Coutinho Sardo
Investigador Auxiliaragradecimentos
The authors are thankful to Dr. A. S. Azevedo for collecting the powder diffraction data. O.M. and R.A. are recipients of fellowships (SFRH/BD/36463/2007, SFRH/BI/51118/2010) from Fundacao para a Ciencia e a Tecnologia (FCT, Portugal). This work was supported by the FCT projects refs PEst-C/QUI/UI0686/2011, PEst-C/CTM/LA0011/2011, and PTDC/SAU-FCF/104347/2008, under the scope of