Intrinsically Disordered Protein Analysis.

abstract

Protein partitioning in aqueous two-phase systems is a technique that enables one to monitor changes in the 3D structures of proteins in solution resulting from chemical modifications, conformational changes, and interactions with other proteins and ligands. The advantage of this technique is that it may be used to monitor the aforementioned changes for purified proteins as well as for proteins in biological fluids, and that it is readily adaptable to automated high-throughput mode.

authors

Mikheeva, LM; Madeira, PP; Zaslavsky, BY

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