Coextração supercrítica do óleo e de diterpenos do resíduo de café


The present work aims to optimize supercritical fluid extraction of spent residue coffee and naturally occurring diterpenes by response surface methodology using Box-Behnken design. The influence of pressure, temperature and cosolvent (ethanol) conditions was studied in the range of 140-190 bar, 40-70ºC and 0-5 % (w/w). Three cumulative extraction curves were measured at 190 bar/40ºC, 190bar/ 55ºC and 140 bar/55ºC, without cosolvent, and allowed the profiles along time of oil and diterpenes extraction to be known. The identification and quantification of most abundant diterpenes (Cafestol, Kahweol and 16-O-methyl-cafestol) was achieved by high performance liquid chromatography (HPLC) analysis, while the triglycerides fatty acids measurement was achieved by gas chromatography (GC). Based on the response surface methodology results, the optimal conditions to maximize oil yield (% w/w) are 190 bar/55ºC/5% (w/w) of ethanol, while maximum diterpenes concentration is obtained at 140 bar/40ºC without ethanol

subject category



Hugo Miguel Amorim Barbosa

our authors


Carlos Manuel Silva; Cláudia Pereira Passos


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